Difference between revisions of "IGEM:Paris Bettencourt 2012/Notebooks/Delay group/day by day//2012/07/31"

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(Entry title)
(Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI))
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Result : only clone 9 shows a band at 2500bp
 
Result : only clone 9 shows a band at 2500bp
  
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[[Image:KR003918.png]]
  
 
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Revision as of 08:29, 7 August 2012

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Results of the double transformation in top 10 cells

Positive control : OK

pBad-aOmpF-7.9 : OK

pKP33-aOmpF-GFPuv : OK

pKP33-GFPuv : OK, no fluo

pBad-aOmpF-7.9 + pKP33-aOmpF-GFPuv : OK, but no fluo

pBad-aOmpF-7.9 + pKP33-GFPuv : no colony

Digestion of the clone 8-9-10 (ligation pLac-RBS-GFP-TT + araC-pBad-RBS-lacI)

We digest 1µg of them in 10µL with EcoRI and PstI (1µL each)

Result : only clone 9 shows a band at 2500bp

KR003918.png