Difference between revisions of "IGEM:NYMU/2010"
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Latest revision as of 02:02, 14 December 2012
SpeedyBac Provide a faster assay system for exploring the design rules of synthetic biology.
Why do we want to do that?
- detect gene expression quantitatively in both space and time.
- specific insight into the flow of genetic information.
- provide speedy ways to report and stop gene expression.
To achieve our specific aim, we have designed a novel reporting device(Speedy reporter) for quickly detectin and measuring the mRNA location and quantity, it can also be used for protein detection. And we design a novel switch (Speedy switch) for control the mRNA translation of gene expression. We have also designed a faster degradation device (Speedy protein degrader); it allows us to regulate the degradation time for studying the mRNAs without the interference from translation and quickly stopping the gene expression.
Our SpeedyBac system is made up of the following three devices:
- Speedy switch
Faster production of protein by inducing the translation of pre-existing mRNA molecules.
- Speedy reporter
Using mRNA aptamers and split GFP-eIF4A reporter designs to detect promoter activity faster.
- Speedy protein degrader
Fast, specific, and constitutive proteolysis achieved by engineering fluorescent proteins tagged with LVA