Difference between revisions of "IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/08"

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(Autocreate 2013/08/08 Entry for IGEM:Missouri_University_of_Science_and_Technology/2009/Notebook/Cyanobacteria_Mediated_Filtration_of_Coal_Flue)
 
(Date: MM/DD/YY People in lab: Yourname)
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== <b>Date: MM/DD/YY</b> <b>People in lab: Yourname</b>  ==
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== <b>Date: 08/08/13</b> <b>People in lab: Blythe Ferriere</b>  ==
  
<b><span style="font-size:16px;">Title:</span></b> Fill in the fields on this page to create a new notebook entry.
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<b><span style="font-size:16px;">Title:</span></b> Blue/white screening for norV TOPO-TA cloned colonies
  
<b>Start Time:</b>
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<b>Start Time:</b> 4:10PM
  
<b>Purpose:</b>  
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<b>Purpose:</b> Plate screened for blue white colonies white colonies should have plasmid.
  
<b>Protocol:</b> <b>Exceptions:</b>
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<b>Protocol:</b> Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes
  
 
<b>Products:</b>  
 
<b>Products:</b>  
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! Sample Label !! Description !! Source Label !! Quantity
 
! Sample Label !! Description !! Source Label !! Quantity
 
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|-
| Entry || Entry || Entry || #
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| 8/8/13 I 1 || Isolation and inoculation of white colony || 8/7/13 CT1 || 8
 
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|-
| Entry || Entry || Entry || #
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| 8/8/13 I 9 || Isolation and inoculation of light blue colony || 8/7/13 CT1 || 1
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|-
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| 8/8/13 I 10 || Isolation and inoculation of blue colony || 8/7/13 CT1 || 3
 
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<b>Results:</b>
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<b>Notes:</b> Ampicillin re-done in I1-I8
 
 
<b>Notes:</b>  
 
  
<b>Stop Time:</b>  
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<b>Stop Time:</b> 5:41 PM
  
<b>Next:</b>
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<b>Next:</b> Pick tubes with growth from white colonies for further analysis for check for norV gene
  
  

Revision as of 13:20, 20 March 2014

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Date: 08/08/13 People in lab: Blythe Ferriere

Title: Blue/white screening for norV TOPO-TA cloned colonies

Start Time: 4:10PM

Purpose: Plate screened for blue white colonies white colonies should have plasmid.

Protocol: Colonies from plate CT1 8/7/13 were inoculated into LB broth + ampicillin tubes

Products:

Sample Label Description Source Label Quantity
8/8/13 I 1 Isolation and inoculation of white colony 8/7/13 CT1 8
8/8/13 I 9 Isolation and inoculation of light blue colony 8/7/13 CT1 1
8/8/13 I 10 Isolation and inoculation of blue colony 8/7/13 CT1 3

Notes: Ampicillin re-done in I1-I8

Stop Time: 5:41 PM

Next: Pick tubes with growth from white colonies for further analysis for check for norV gene