IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/07

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Revision as of 09:54, 11 February 2014 by Levi Palmer (talk | contribs) (Date: 08/07/13 People in lab: Blythe Ferriere)
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Date: 08/07/13 People in lab: Blythe Ferriere

Title: Gel Electrophoresis of 8/6/13 A1-A6 PCR reactions

Start Time: 8:45 AM

Purpose: To check for norV gene product with prefix&suffix from PCR amplification.

Protocol: LTM ed. 2 pg 45-47

Well 1 2 3 4 5 6 7 8
Sample Ladder 8/6/13 A1 8/6/13 A2 8/6/13 A3 8/6/13 A4 8/6/13 A5 8/6/13 A6 Ladder

Products: N/A

Results: PICTURE


Stop Time: 12:33 PM

Next: TOPO cloning of 8/6/13 PCR products

Date: 08/07/13 People in lab: Blythe Ferriere

Title: TOPO cloning of PCR product and chemical transformation

Start Time: 2:45 PM

Purpose: To TOPO clone 8/6/13 A1 norV PCR product and chemically transform to amplify gene product

Protocol: TOPO cloning: Fresh PCR product 4uL, PCR TOPO vector 1uL. 1. Mix gently and incubate for 5 minutes at room temperature. Transformation: LTM ed. 2 pg. 42 Exceptions: 1. 2uL of TOPO cloning reaction product 2. 1uL pUC19 control standard 3. Plate on amp+Xgal


Notes: PCR product was a day old. 1 hour 35 min recovery time.

Stop Time: 3:40 PM

Next: TOPO cloning of 8/6/13 PCR products