IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/03

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Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (1/2)

Title: Gel electrophoresis of 8/2/13 A norCB 1 and 2

Start Time: 2:05 PM

Purpose: Confirm norCB gene with iGEM prefix & suffix. Use the better sample to purify and sequence.

Protocol: LTM ed. 2 pg 45-47


Well 1 2 3 4 5 6 7 8
Sample Ladder 8/2/13 A norCB 1 8/2/13 A norCB 2

Products: N/A

Results: PICTURE

Notes:

Stop Time: 3:30 PM

Next: The ~1900 bp fragment from 8/2/13 A norCB 2 should be extracted and purified.


Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (2/2)

Title: Gel extraction of ~2000bp fragment from today's gel

Start Time: 3:50 PM

Purpose: To extract the PCR product of the norCB gene + iGEM prefix & suffix

Protocol: IBI Gel extraction Exceptions: 1. Milli-Q used instead of Elution Buffer

Products:

Sample Label Description Source Label Quantity
8/3/13 GE norCB norCB w/ iGEM prefix/suffix (~1900 bp) from well 3 Well 3 8/3/13 Gel 1

Results: N/A

Notes:

Stop Time: 4:45 PM

Next: 8/3/13 GE norCB should be ligated into the iGEM vector.