IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/03: Difference between revisions
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span> | |style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== <b>Date: 08/3/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b> == | == <b>Date: 08/3/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b> (1/2) == | ||
<b><span style="font-size:16px;">Title:</span></b> Gel electrophoresis of 8/2/13 A norCB 1 and 2 | <b><span style="font-size:16px;">Title:</span></b> Gel electrophoresis of 8/2/13 A norCB 1 and 2 | ||
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== <b>Date: 08/3/13</b> <b>People in lab: Emily Puleo, Kelsey Crossen</b> (2/2) == | |||
<b><span style="font-size:16px;">Title:</span></b> Gel extraction of ~2000bp fragment from today's gel | |||
<b>Start Time:</b> 3:50 PM | |||
<b>Purpose:</b> To extract the PCR product of the norCB gene + iGEM prefix & suffix | |||
<b>Protocol:</b> IBI Gel extraction <b>Exceptions:</b> 1. Milli-Q used instead of Elution Buffer | |||
<b>Products:</b> | |||
{| border="1" cellpadding="5" | |||
|- | |||
! Sample Label !! Description !! Source Label !! Quantity | |||
|- | |||
| 8/3/13 GE norCB || norCB w/ iGEM prefix/suffix (~1900 bp) from well 3 || Well 3 8/3/13 Gel ||1 | |||
|- | |||
|} | |||
<b>Results:</b> N/A | |||
<b>Notes:</b> | |||
<b>Stop Time:</b> 4:45 PM | |||
<b>Next:</b> 8/3/13 GE norCB should be ligated into the iGEM vector. | |||
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Latest revision as of 23:40, 26 September 2017
 Cyanobacteria Mediated Filtration of Coal Flue Gas
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Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (1/2)Title: Gel electrophoresis of 8/2/13 A norCB 1 and 2 Start Time: 2:05 PM Purpose: Confirm norCB gene with iGEM prefix & suffix. Use the better sample to purify and sequence. Protocol: LTM ed. 2 pg 45-47
Products: N/A Results: PICTURE Notes: Stop Time: 3:30 PM Next: The ~1900 bp fragment from 8/2/13 A norCB 2 should be extracted and purified.
Date: 08/3/13 People in lab: Emily Puleo, Kelsey Crossen (2/2)Title: Gel extraction of ~2000bp fragment from today's gel Start Time: 3:50 PM Purpose: To extract the PCR product of the norCB gene + iGEM prefix & suffix Protocol: IBI Gel extraction Exceptions: 1. Milli-Q used instead of Elution Buffer Products:
Results: N/A Notes: Stop Time: 4:45 PM Next: 8/3/13 GE norCB should be ligated into the iGEM vector. | |||||||||||||||||||||||||||
