Difference between revisions of "IGEM:Missouri University of Science and Technology/2009/Notebook/Cyanobacteria Mediated Filtration of Coal Flue/2013/08/02"

From OpenWetWare
Jump to: navigation, search
(Date: MM/DD/YY People in lab: Yourname)
(fix raw html notebook nav)
 
Line 2: Line 2:
 
|-
 
|-
 
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
 
|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:20px;"> Cyanobacteria Mediated Filtration of Coal Flue Gas</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
+
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
|-
 
|-
 
| colspan="2"|
 
| colspan="2"|

Latest revision as of 22:40, 26 September 2017

Igem-logo-150px.png Cyanobacteria Mediated Filtration of Coal Flue Gas Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Date: 08/02/13 People in lab: Emily Puleo, Kelsey Crossen

Title: PCR of norCB

Start Time: 4:30 PM

Purpose: To PCR norCB and add iGEM prefix&suffix

Protocol: LTM ed. 2 pg 45-47 Exceptions: 1. Final primer concentration <0.1 uM 2. Decreased annealing temperature to 63C 3. Increased elongation time to 5 minutes

Products:

Sample Label Description Source Label Quantity
8/2/13 A norCB 1 norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa P. aeruginosa 7/30 1
8/2/13 A norCB 2 norCB gene from P. aeruginosa with iGEM prefix and suffix using 7.5 uL template P. aeruginosa 2x concentration P. aerguginosa 7/30 2x in H2O 1

Results: N/A

Notes:

Stop Time: 8:35 PM

Next: Run samples on a gel. Extract, purify, sequence the better resultant gene product.