Difference between revisions of "IGEM:MIT/2008/Notebook/Yogurt"

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* [[../Yogurt/sequences |Primers/Plasmids/PCR sequences]]
 
* [[../Yogurt/sequences |Primers/Plasmids/PCR sequences]]
  
* [[../Yogurt/lbulgaricus |''L. delbrueckii subsp. bulgaricus'' information & protocols]]
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* [[../Yogurt/lbulgaricus |''Lactobacillus delbrueckii'' subsp. ''bulgaricus'' Information & Protocols]]
  
 
==Useful Links==
 
==Useful Links==

Revision as of 09:01, 18 June 2008

<!-- sibboleth --><div id="lncal1" style="border:0px;"><div style="display:none;" id="id">lncal1</div><div style="display:none;" id="dtext">06/16/2008,06/17/2008,06/20/2008,06/23/2008</div><div style="display:none;" id="page">IGEM:MIT/2008/Notebook/Yogurt</div><div style="display:none;" id="fmt">yyyy/MM/dd</div><div style="display:none;" id="css">OWWNB</div><div style="display:none;" id="month"></div><div style="display:none;" id="year"></div><div style="display:none;" id="readonly">Y</div></div>

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Description

  • Our goal is to engineer a common yogurt bacteria, Lactobacillus bulgaricus, so that it will express the 20aa peptide p1025. A clinical study (Kelly CG et al.; Nature Biotechnol. 1999) reports that p1025 is good for your teeth. p1025 reduces oral colonization of Streptococcus mutans, a tooth-decaying bacterium.
    • Stage 1: Construction of p1025 fusion peptide and expression of gene in E. coli. This is an intermediate step to evaluate gene function and protein secretion/efficacy.
    • Stage 2: Binding Assay - see if the p1025 produced by E.coli inhibits binding of S. mutants to hydroxyapatite (HA) beads.
    • Stage 3: Expression of p1025 in Lactobacillus.
    • Stage 4: Make yogurt with modified Lactobacillus and test for inhibition of S. mutants binding.

Notes

  • Group meetings every Friday at 4pm in 68-574

Information

  • [[../Yogurt/Brainstorming |Brainstorming]]
  • [[../Yogurt/Notes |Daily Notebook]]
  • [[../Yogurt/sequences |Primers/Plasmids/PCR sequences]]
  • [[../Yogurt/lbulgaricus |Lactobacillus delbrueckii subsp. bulgaricus Information & Protocols]]

Useful Links

  • |DNA Plasmid Editor
  • |Peptide Sequence Manipulations
  • Primer Analyzer Tool (check temperature & GC content) | One | Two
  • PMID 9920267
    A synthetic peptide adhesion epitope as a novel antimicrobial agent.
  • PMID 9062560
    Inhibition of Streptococcus mutans adsorption to hydroxyapatite by low-molecular-weight chitosans.
  • PMID 12788739
    (a secretion signal sequence described; can use the promoter, RBS and secretion signal for this protein for p1025).
  • PMID 11772607
    Electrotransformation of Lactobacillus delbrueckii subsp. bulgaricus and L. delbrueckii subsp. lactis with various plasmids.