Difference between revisions of "IGEM:MIT/2007/Notebook/2007-8-11"

From OpenWetWare
Jump to: navigation, search
(New page: ==Agenda== #Double digestion: #*R0051.B0034, I14032.B0034, CPX.B0014 - for 3A assembly into pSB1AC3 #*F2620.B0034.CPX, B0014 - for standard assembly and to figure out extra bands in wester...)
(No difference)

Revision as of 08:13, 11 August 2007

Agenda

  1. Double digestion:
    • R0051.B0034, I14032.B0034, CPX.B0014 - for 3A assembly into pSB1AC3
    • F2620.B0034.CPX, B0014 - for standard assembly and to figure out extra bands in western blot
  2. Purify digestion reactions. Remember to use BUFFER TE<b> and NOT H2O.
  3. Ligations of digestion mixtures
    • R0051.B0034.CPX.B0014
    • I14032.B0034.CPX.B0014
    • F2620.B0034.CPX.B0014
  4. Transform ligations into DH5a and plate.