Difference between revisions of "IGEM:MIT/2007/Notebook/2007-8-1"

From OpenWetWare
Jump to: navigation, search
(Flourescence Assay Protocol)
(Flourescence Assay Protocol)
Line 15: Line 15:
# Put 150ul in plate reader
# Put 150ul in plate reader
Image:8-1-AHL-5Ai.jpg|AHL 5Ai
Image:8-1-AHL-5Ai.jpg|AHL 5Ai

Revision as of 07:08, 2 August 2007


  1. Decide on constitutive promoter for CPX
    • Transform promoter into DH5a
  2. Check sequencing results for Mer/GFP 3A
  3. Perform flourescence assay on mer-gfp construct

Flourescence Assay Protocol

  1. Draw sample of 1.5 ml from incubated cells with Hg
  2. Plot OD's
  3. Spin down 3 mins at 13000 rpm
  4. Aspirate and resuspend in 1.5 ml .9% NaCl
  5. Repeat 3
  6. Repeat 4
  7. Put 150ul in plate reader


Sequencing Results

  • mer.I13500 in pSB1AT3 came back really crappy. Biobrick prefix and suffix are partially present, cannot locate the sequence for GFP.
  • Need to send in the parts for sequencing.
  • Will run a colony PCR as a diagnostic.