IGEM:MIT/2007/Notebook/2007-7-30: Difference between revisions
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(New page: ==Mer-GFP Expression Protocol== # Mix Diluted LB Media (LB4.10) ##4g NaCl, .5g Yeast Exract, 1g Tryptone + 1L H20 # Autoclave LB4.10 # Grow up 10 mls ON at 30°C # Dilute back 1:100 in fr...) |
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Revision as of 09:30, 30 July 2007
Mer-GFP Expression Protocol
- Mix Diluted LB Media (LB4.10)
- 4g NaCl, .5g Yeast Exract, 1g Tryptone + 1L H20
- Autoclave LB4.10
- Grow up 10 mls ON at 30°C
- Dilute back 1:100 in fresh LB4.10
- Add HgCl2 in following concentrations (ng/ml):
- 0
- 5
- 10
- 25
- 50
- 75
- 100
- 150
- 300
- 500
- 1000
- 10000
- Grow at 30°C for 16h