IGEM:MIT/2007/Notebook/2007-7-27: Difference between revisions
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Image:Image21.jpg|Image 21 | Image:Image21.jpg|Image 21 | ||
</gallery> | </gallery> | ||
[[Image:Polystyreneassay.jpg|thumb|Diagram of the Polystyrene Assay Protocol]] | |||
==Western Blot Results== | ==Western Blot Results== | ||
[[Media:070727_iGEM2007_t7_fusions.pdf|A pdf of the blots]] | [[Media:070727_iGEM2007_t7_fusions.pdf|A pdf of the blots]] | ||
Revision as of 10:17, 17 August 2007
Agenda
- dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
- Miniprep LC's of Mer/GFP
- Finish Western Blot
- Order EC20 oligos
EC_20 Oligos
- Ordered with IE-HCLG purification, should arrive Tuesday
Polystyrene Assay
Wells: (Done in Triplicate) CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)
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E-5a
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E-5b
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E-5c
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E-6a
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E-6b
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E-6c
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E-7a
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E-7b
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E-7c
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E-7d
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E-7e
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E-7f
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E-8a
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E-8b
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E-8c
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E-8d
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E-8e
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E-8f
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Control 1
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Control 2
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Control 3
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Image 21
