IGEM:MIT/2007/Notebook/2007-7-27: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 8: Line 8:
* Ordered with IE-HCLG purification, should arrive Tuesday
* Ordered with IE-HCLG purification, should arrive Tuesday


==Polystyrene Assa=
==Polystyrene Assay==


<pre>
<pre>

Revision as of 12:36, 27 July 2007

Agenda

  • dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
    • Miniprep LC's of Mer/GFP
  • Finish Western Blot
  • Order EC20 oligos

EC_20 Oligos

  • Ordered with IE-HCLG purification, should arrive Tuesday

Polystyrene Assay

Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)