IGEM:MIT/2007/Notebook/2007-7-27: Difference between revisions
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(New page: ==Agenda== *dilute LC's of Mer/GFP Transformations and BL21 untransformed cells **Miniprep LC's of Mer/GFP *Finish Western Blot *Order EC20 oligos) |
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=Agenda= | |||
*dilute LC's of Mer/GFP Transformations and BL21 untransformed cells | *dilute LC's of Mer/GFP Transformations and BL21 untransformed cells | ||
**Miniprep LC's of Mer/GFP | **Miniprep LC's of Mer/GFP | ||
*Finish Western Blot | *Finish Western Blot | ||
*Order EC20 oligos | *Order EC20 oligos | ||
==EC_20 Oligos== | |||
* Ordered with IE-HCLG purification, should arrive Tuesday | |||
==Polystyrene Assa= | |||
<pre> | |||
Wells: | |||
(Done in Triplicate) | |||
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced) | |||
Revision as of 12:36, 27 July 2007
Agenda
- dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
- Miniprep LC's of Mer/GFP
- Finish Western Blot
- Order EC20 oligos
EC_20 Oligos
- Ordered with IE-HCLG purification, should arrive Tuesday
=Polystyrene Assa
Wells: (Done in Triplicate) CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)
