Difference between revisions of "IGEM:MIT/2007/Notebook/2007-7-27"

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(New page: ==Agenda== *dilute LC's of Mer/GFP Transformations and BL21 untransformed cells **Miniprep LC's of Mer/GFP *Finish Western Blot *Order EC20 oligos)
 
(Agenda)
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==Agenda==
+
=Agenda=
 
*dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
 
*dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
 
**Miniprep LC's of Mer/GFP
 
**Miniprep LC's of Mer/GFP
 
*Finish Western Blot
 
*Finish Western Blot
 
*Order EC20 oligos
 
*Order EC20 oligos
 +
 +
==EC_20 Oligos==
 +
* Ordered with IE-HCLG purification, should arrive Tuesday
 +
 +
==Polystyrene Assa=
 +
 +
<pre>
 +
Wells:
 +
(Done in Triplicate)
 +
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)

Revision as of 12:36, 27 July 2007

Agenda

  • dilute LC's of Mer/GFP Transformations and BL21 untransformed cells
    • Miniprep LC's of Mer/GFP
  • Finish Western Blot
  • Order EC20 oligos

EC_20 Oligos

  • Ordered with IE-HCLG purification, should arrive Tuesday

=Polystyrene Assa

Wells:
(Done in Triplicate)
CPX(-5 AHL) CPX(-6 AHL) CPX(-7 AHL) CPX(-8 AHL) BLANK WELL PICCS8(1/500) Neg Control(Uninduced)