IGEM:MIT/2007/Ideas3: Difference between revisions
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===Sequence=== | ===Sequence=== | ||
#Native SS | #Native SS <pre> MKKIACLSALAAVLAFTAGTSVA </pre> | ||
<pre> | #Embedded SfiI restriction site <pre> GQSGQ </pre> | ||
MKKIACLSALAAVLAFTAGTSVA | #Passenger Peptide <pre> XXXXXXXXXXXXXX </pre> | ||
</pre> | #Sequence <pre> GGQSGQ </pre> | ||
#Embedded SfiI restriction site | #S54-F148 <pre> SGDYNKNQYYGITAGPAYRINDWASIYGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVGTWIAGVGYRF </pre> | ||
<pre> | #Join native C and N <pre> GGSG </pre> | ||
GQSGQ | #A1-S53 <pre> ATSTVTGGYAQSDAQGQMNKMGGFNLKYRYEEDNSPLGVIGSFTYTEKSRTAS </pre> | ||
</pre> | |||
#Passenger Peptide | |||
<pre> | |||
XXXXXXXXXXXXXX | |||
</pre> | |||
#Sequence | |||
<pre> | |||
GGQSGQ | |||
</pre> | |||
#S54-F148 | |||
<pre> | |||
SGDYNKNQYYGITAGPAYRINDWASIYGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVGTWIAGVGYRF | |||
</pre> | |||
Join native C and N | |||
GGSG | |||
A1-S53 | |||
ATSTVTGGYAQSDAQGQMNKMGGFNLKYRYEEDNSPLGVIGSFTYTEKSRTAS | |||
==Papers!== | ==Papers!== | ||
Revision as of 12:32, 18 June 2007
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Metal Pumps and Metal Specific Promoters
Mussel Protein
Expressing proteins on E coli cell membranes
Water Purification Methods
Metal Pumps and Metal Specific Promoters
Mussel Protein
Water Purification Methods
OmpX
Sequence
- Native SS
MKKIACLSALAAVLAFTAGTSVA
- Embedded SfiI restriction site
GQSGQ
- Passenger Peptide
XXXXXXXXXXXXXX
- Sequence
GGQSGQ
- S54-F148
SGDYNKNQYYGITAGPAYRINDWASIYGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDVGTWIAGVGYRF
- Join native C and N
GGSG
- A1-S53
ATSTVTGGYAQSDAQGQMNKMGGFNLKYRYEEDNSPLGVIGSFTYTEKSRTAS
Papers!
- Samuelson P, Wernérus H, Svedberg M, and Ståhl S. Staphylococcal surface display of metal-binding polyhistidyl peptides. Appl Environ Microbiol. 2000 Mar;66(3):1243-8. DOI:10.1128/AEM.66.3.1243-1248.2000 |
- Kotrba P, Dolecková L, de Lorenzo V, and Ruml T. Enhanced bioaccumulation of heavy metal ions by bacterial cells due to surface display of short metal binding peptides. Appl Environ Microbiol. 1999 Mar;65(3):1092-8. DOI:10.1128/AEM.65.3.1092-1098.1999 |
