Difference between revisions of "IGEM:MIT/2006/Notebook/2006-8-3"

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(IAA Digest)
Line 34: Line 34:
#THI3 with SX
#THI3 with SX
#BKB with SX
#BKB with SX
==IAA Ligations==
#BAT2<sub>EP</sub> w/ A/C & A/T BKB
#THI3<sub>SX</sub> w/ psBIAK3<sub>SX</sub>->A/K plate

Revision as of 14:57, 2 August 2006

Primer To Do

  1. Order new pchBA primers

Miniprep/Digest To Do

  1. test osmY-E0840 with plate reader
  2. Miniprep BSMT.B0015 LC
  3. Cut BSMT.B0015 with XP
  4. Miniprep B0030
  5. Cut B0030 with ES and SP(bkb)
  6. Miniprep R0040
  7. Cut R0040 with ES and SP(bkb)

WG Ligations/Transformations To Do

  1. Gel extract BSMT.B0015XP
  2. Gel extract B0030SP backbone
  3. Ligate BSMT.B0015XP and B0030SP backbone-> transform

  1. Ligate B0030ES with BSMT.B0015XP in A/CEP
  2. Ligate B0030ES with BSMT.B0015XP in A/TEP

osmY Ligations/Transformations To Do

  1. osmY and A/CEP and A/TEP

ATF1 Miniprep/Digest To Do

  1. Miniprep ATF1.B0015 LC
  2. Cut ATF1.B0015 with XP
  3. Gel extract some of ATF1.B0015XP

ATF1 Ligation/Transformation To Do

  1. Ligate ATF1.B0015XP with B0030ES in A/CEP and A/TESP

IAA Digest

  1. BAT2 with EP, ligate with A/C and A/TEP
  2. THI3 with SX
  3. BKB with SX

IAA Ligations

  1. BAT2EP w/ A/C & A/T BKB
  2. THI3SX w/ psBIAK3SX->A/K plate