IGEM:MIT/2006/Notebook/2006-8-3: Difference between revisions
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#Cut BSMT.B0015 with XP | #Cut BSMT.B0015 with XP | ||
#Miniprep B0030 | #Miniprep B0030 | ||
#Cut B0030 with ES | #Cut B0030 with ES and SP(bkb) | ||
#Miniprep R0040 | |||
#Cut R0040 with ES and SP(bkb) | |||
==WG Ligations/Transformations To Do== | ==WG Ligations/Transformations To Do== | ||
Revision as of 14:08, 2 August 2006
to do
- test osmY-E0840 with plate reader
- Miniprep BSMT.B0015 LC
- Cut BSMT.B0015 with XP
- Miniprep B0030
- Cut B0030 with ES and SP(bkb)
- Miniprep R0040
- Cut R0040 with ES and SP(bkb)
WG Ligations/Transformations To Do
- Gel extract BSMT.B0015XP
- Gel extract B0030SP backbone
- Ligate BSMT.B0015XP and B0030SP backbone-> transform
- Ligate B0030ES with BSMT.B0015XP in A/CEP
- Ligate B0030ES with BSMT.B0015XP in A/TEP
osmY Ligations/Transformations To Do
- osmY and A/CEP and A/TEP
