IGEM:MIT/2006/Introduction: Difference between revisions
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This page contains links to introductory information for the MIT iGEM team. | This page contains links to introductory information for the MIT iGEM team. | ||
== | ==Design== | ||
===System Design considerations=== | |||
*Feasible system scale (numbers of parts, plasmids, chassis types) | |||
*Design tools - models, network diagrams | |||
*Moving from system->devices->parts->DNA specification | |||
*Likelihood of success - protein engineering, cloning, new biological techniques and new science etc. | |||
===Abstraction hierarchy & standardization=== | |||
*Parts - zinc fingers? | |||
*Devices - receiver?, inverter? | |||
*Systems - bacterial photography? | |||
== | |||
* | |||
* | |||
*PoPS and composability | *PoPS and composability | ||
== | ==Build== | ||
===Construction methods=== | |||
== | |||
*[[BioBricks]] <font color=red>- Day 1, Week 1</font> | *[[BioBricks]] <font color=red>- Day 1, Week 1</font> | ||
*Standard assembly <font color=blue>- Day 2, Week 1</font> | *Standard assembly <font color=blue>- Day 2, Week 1</font> | ||
| Line 31: | Line 22: | ||
*Direct synthesis | *Direct synthesis | ||
== | ===Construction tools=== | ||
*Registry | |||
*#Obtain accounts <font color=red>- Day 1, Week 1</font> | |||
*#Making a part (both basic and composite) <font color=red>- Day 1, Week 1</font> | |||
*#BLAST | |||
*#Sequence alignments | |||
*NCBI Entrez <font color=red>- Day 1, Week 1</font> | |||
*VectorNTI | |||
*Others? | |||
==Test== | |||
*Plate reader characterization (i.e. Receiver) | *Plate reader characterization (i.e. Receiver) | ||
*Flow cytometry (i.e. Screening plasmid) | *Flow cytometry (i.e. Screening plasmid) | ||
| Line 37: | Line 38: | ||
*RT-PCR and Westerns | *RT-PCR and Westerns | ||
== | ==Necessary skills== | ||
*[[ | ===Lab notebooks & documentation=== | ||
*[[OpenWetWare:Getting started | Getting started with wiki editing]] <font color=red>- Day 1, Week 1</font> | |||
*Populate the [http://parts.mit.edu/wiki/index.php/MIT_2006 official MIT iGEM 2006 wiki page] | |||
*The online lab notebook framework | |||
==Lab | ===Searching and reading the literature=== | ||
*[[Searching the literature]] <font color=green>- Day 3, Week 1</font> | |||
===Lab safety=== | |||
*Complete the [[Endy:Safety_Training | '''Endy lab safety training''']]. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab. | |||
*Heather will be giving the lab-specific training <font color=red>- Day 1, Week 1</font> | |||
==Links== | ==Links== | ||
'''General introduction to ideas in Synthetic Biology''' | '''General introduction to ideas in Synthetic Biology''' | ||
#[[Adventures | Adventures in synthetic biology comic]] --> Learn about PoPS | #[[Adventures | Adventures in synthetic biology comic]] --> Learn about PoPS | ||
#[http://openwetware.org/images/0/0d/Nature04342.pdf Foundations for engineering biology] --> rant by Drew Endy | #[http://openwetware.org/images/0/0d/Nature04342.pdf Foundations for engineering biology] --> rant by Drew Endy | ||
| Line 51: | Line 59: | ||
#Find descriptions of [http://parts2.mit.edu/wiki/index.php/Igem_2005 2005 projects] | #Find descriptions of [http://parts2.mit.edu/wiki/index.php/Igem_2005 2005 projects] | ||
== | ==Schedules== | ||
*[[IGEM:MIT/2006/Introduction/Week 1]] | |||
* | |||
Latest revision as of 12:14, 2 June 2006
This page contains links to introductory information for the MIT iGEM team.
Design
System Design considerations
- Feasible system scale (numbers of parts, plasmids, chassis types)
- Design tools - models, network diagrams
- Moving from system->devices->parts->DNA specification
- Likelihood of success - protein engineering, cloning, new biological techniques and new science etc.
Abstraction hierarchy & standardization
- Parts - zinc fingers?
- Devices - receiver?, inverter?
- Systems - bacterial photography?
- PoPS and composability
Build
Construction methods
- BioBricks - Day 1, Week 1
- Standard assembly - Day 2, Week 1
- 3A assembly
- In frame assembly
- Direct synthesis
Construction tools
- Registry
- Obtain accounts - Day 1, Week 1
- Making a part (both basic and composite) - Day 1, Week 1
- BLAST
- Sequence alignments
- NCBI Entrez - Day 1, Week 1
- VectorNTI
- Others?
Test
- Plate reader characterization (i.e. Receiver)
- Flow cytometry (i.e. Screening plasmid)
- Microscopy
- RT-PCR and Westerns
Necessary skills
Lab notebooks & documentation
- Getting started with wiki editing - Day 1, Week 1
- Populate the official MIT iGEM 2006 wiki page
- The online lab notebook framework
Searching and reading the literature
- Searching the literature - Day 3, Week 1
Lab safety
- Complete the Endy lab safety training. (Even if you have worked in a lab before, you should visit this page and verify that your training is up to date.) This should be done before your first day in lab.
- Heather will be giving the lab-specific training - Day 1, Week 1
Links
General introduction to ideas in Synthetic Biology
- Adventures in synthetic biology comic --> Learn about PoPS
- Foundations for engineering biology --> rant by Drew Endy
- MIT iGEM 2004 presentation (.ppt,pdf)
- UT Austin and UCSF 2004 project (coliroids)
- Find descriptions of 2005 projects
