Difference between revisions of "IGEM:MIT/2006/Communications"

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(osmY Promoter Mehods Section (In order))
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===Figure 1===
Complete system diagram.
Please ask [[User:Reshma P. Shetty|Reshma]] for the latest copy of the paper and figures.
===Figure 2===
Smell generating devices from precursor. 
*(a) device diagrams of wintergreen and banana generating devices.
**[[Image:MITiGEM2006Figure2a.pdf|Figure 2a]]
*(b) smell test results from Jamboree
**[[Image:IGEM06Smelltest.xls|Jamboree smell test data]] (xls)
*(c) smell test photo?
===Figure 3===
Autonomous wintergreen production
*(a) Device and parts level diagram of autonomous wintergreen synthesis
*(b) GC data of wintergreen total synthesis
*(c) GC data of J45120 without precursor
*(d) Pure methyl salicylate standard
===Figure 4===
Growth phase control
*(a) parts and device level depiction
*(b) growth-phase control of FP output
===Figure 5===
*(a) device diagram of regulated banana device
*(b) timecourse of banana smell
===Figure S1===
Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].
===Figure S2===
*(a) GC data for IK cells versus normal E. coli;
*(b) mint scented bacteria with precursor versus TOP10 with precursor.
*(c) banana scented bacteria with precursor versus TOP10 with precursor.
Also see the relevant part pages for references.
===osmY Promoter Mehods Section (In order)===
book reference:
Chasel, M. Gentry D.R., Hernandez, V.J., and Vinella, D. (1996) in Neidhardt, F.C. (ed.) ''Escherichia coli and Salmonella-Cellular and Molecular Biology'', 2nd edition. pp. 1476-1477, ASM Press, Washington, DC.
#Neidhardt isbn=1555810845
#Colland-EMBO-2000 pmid=10856246
#Schelhorn pmid=9829938
#Yim pmid=8282684
===Odor thresholds===
===Odor thresholds===
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*[http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf Isoamyl acetate]
*[http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf Isoamyl acetate]
*[http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362 Methyl salicylate]
*[http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362 Methyl salicylate]
===Use of ATF1 in ''E. coli''===
#Singh-BioprocessBiosysEng-2007 pmid=17891501
===Methyl salicylate synthesis===
#Negre-ArchBiochemBiophys-2002 pmid=12361714
#Ross-ArchBiochemBiophys-1999 pmid=10375393
#Pott-PlantPhysiol-2004 pmid=15310828
#Zubieta-PlantCell-2003 pmid=12897246

Revision as of 14:11, 23 February 2008

Please ask Reshma for the latest copy of the paper and figures.

Odor thresholds

"The odor threshold of a compound is the lowest concentration at which its smell can be detected."