IGEM:MIT/2006/Communications

Figure 1

• (a) normal e. coli (depict chemical inputs and outputs)
• (b) final eau d’e coli system (depict chemical inputs and outputs)

Figure 1 (pdf)

Figure 2

Device depictions (show one or two devices at the parts level, perhaps also show a device transfer function and also whatever the most relevant device data is, perhaps the GC data)

Figure 2 (pdf)

Figure 3

GC data (banana total synthesis, wintergreen total synthesis, banana with precursor, wintergreen with precursor)

Figure 4

Smell test data (show photo of smell test in action and also bar graphs of results)

Jamboree smell test data (xls)

Bar graph of smell test results (pdf)

Figure 5

Growth phase control (parts and device level depiction)

Figure 5 (pdf)

• verify the part numbers of the devices for which there is GC data ...

J45993 vs J45992 and J45199 vs J45219

Figure 6

Show data with growth-phase control of FP output

• ask Barry to make pretty figure for this data

Figure 7

Show GC data from banana time course

Figure S1

Restrospective project timeline. How long did each step take? [from this we can compute how we could do such work faster. We’ll want to work the pace of work into both the introduction and discussion].

Figure S2

Show GC data here (could have chromatograms)? GC data for IK cells versus normal E. coli; mint scented bacteria versus IK cells.

References

Paper demonstrating successful transfer of ATF1 into E. coli:

http://www.ncbi.nlm.nih.gov/pubmed/17891501?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum

"The odor threshold of a compound is the lowest concentration at which its smell can be detected." Isoamyl acetate's odor threshold listed here:

http://people.ok.ubc.ca/neggers/Chem422A/The%20Odor%20and%20Aroma%20of%20Wine.pdf

Methyl salicylate's odor threshold listed here:

http://www.sciencelab.com/xMSDS-Methyl_salicylate-9927362