IGEM:MIT/2005/Experiments

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[[../Experimental Flowchart/]]
Check.gif indicates done!
Pending.gif indicates pending...

Experiment Status

Stock-up MiniPreps
   Check.gif grow up B0015, J13002, E0840
   Check.gif overnight B0015, J13002, E0840
   Check.gif miniprep B0015, J13002, E0840
   Check.gif glycerol stock J13002


FecA (full length)
   Check.gif PCR bb ends
   Check.gif digest FecA + pSB1AK3-1 plasmid w/ E/S
   Check.gif gel purify each
   Check.gif ligate together
   Check.gif transform
   Check.gif overnights for miniprep
   Check.gif identify correct clones
   Check.gif overnights for freezer stock
   Check.gif freezer stocks

Jessica

MalE
   Check.gif PCR
   Check.gif digest MalE + pSB1AK3-1 plasmid w/ E/S 
   Check.gif gel purify each
   Check.gif ligate together
   Pending.gif transform
   Pending.gif identify correct clones
PhoA
   Check.gif PCR with higher annealing temperature
   Check.gif digest PhoA+ pSB1AK3-1 plasmid w/ E/S 
   Check.gif gel purify each
   Check.gif ligate together
   Pending.gif transform
   Pending.gif identify correct clones

PhoA'
   Pending.gif QuikChange

Annie

FecA promoter
   Check.gif PCR with current primers with higher annealing temperature
   Pending.gif PCR with newly designed primers
   Pending.gif digest FecApro + pSB1AK3-1 plasmid w/ E/S 
   Pending.gif gel purify each
   Pending.gif ligate together
   Pending.gif transform
   Pending.gif identify correct clones (digest)
   Pending.gif freezer stock
FecI
   Check.gif PCR bb end
   Check.gif digest FecI + pSB1AK3-1 plasmid w/ E/S
   Check.gif gel purify each
   Check.gif ligate together
   Check.gif transform
   Pending.gif identify correct clones (digest)
   Pending.gif freezer stock
FecR
   Check.gif PCR bb end
   Check.gif digest FecR + pSB1AK3-1 plasmid w/ E/S
   Check.gif gel purify each
   Check.gif ligate together
   Check.gif transform
   Pending.gif identify correct clones (digest)
   Pending.gif freezer stock
FecA'
   Pending.gif QuikChange
FecR'
   Pending.gif QuikChange

Project Experiments

  Input Device: [[../Input: Ligand#Experiments/|fluorescein]]
  Head Receiver Unit: [[../Receiver Head Unit: scFv#Experiments/|anti-fluorescein scFv]]
  Receiver 1: [[../Receiver 1: ToxR#Experiments/|ToxR]]
  Receiver 2: [[../Sensor 2#Experiments/|FecA]] 
  Signal Processor: [[../Signal processing#Experiments/|Exp. Here]]
  Actuator: [[../Actuator#Experiments/|Exp. Here]]

[[../Experimental Timeline/]]