Difference between revisions of "IGEM:Imperial/2010/Strategy"

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==Wolfs checklist==
==Wolfs checklist==
[[Image:PrimersPic1.bmp|thumb|right|600px|x400px| '''Additional primers - work in progress''']]
* Urgent primers have been ordered on Monday 16th.  
* Urgent primers have been ordered on Monday 16th.  

Revision as of 09:29, 17 August 2010


Assembly strategy and Lab-work

Please refer to the overall assembly strategy for details.

Three Lab-teams are currently working in parallel to assemble parts which will be needed for down-stream steps, as soon as the synthesized sequences arrive.

LINKS to lab teams.

LINKS to shedule history (?)

Wolfs checklist

Additional primers - work in progress
  • Urgent primers have been ordered on Monday 16th.
  • 14 other primers will be required. Design is in progress (pic of word table)!
  • Experimental design for XylE e.coli testing!
  • Functional testing strategies have been developed and have been indicated in the overall assembly strategy diagram. Need to be written up properly!
  • XylE and TEV will not be tagged. In order to be able to analyse their activity, we will compare substrate-turnover rates.
  • Strategies for testing of expression etc have to be developed and structured.
  • List of parts for submission has to be assembled.
  • Gant chart!
  • How much synthetic AIP will be required to trigger our test system? (Modellers)
  • ComE promoter system - recap on mode of function.