IGEM:Imperial/2010/Output module

From OpenWetWare
Jump to: navigation, search
Effector 1 (Protease) Effector 2 (Dye,Enz) Pigment biosynthetic pathways
transcr sigma 54 2 colourless Bilins
Activation (phosphorylation) Enz-in-pathway
  • short pathways
  • ensure - colourless -> colour
  • short pathways
  • ensure - colourless -> colour
Target proteases to use
  • not many AA
  • non-toxic
  • can work in E.Coli
  • quantize speed, efficiency
Protein scaffold Fret pairs as back up for effectors
2C DNA binding prot release

This review article has some useful information on FRET.

James- some suggested papers:

  1. Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 | PubMed ID:17117150 | HubMed [1]
  2. Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 | PubMed ID:17072307 | HubMed [2]
All Medline abstracts: PubMed | HubMed

GFP as output

In vivo and in vitro protein solubility assays using split GFP [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4HKMM92-3&_user=217827&_coverDate=01%2F13%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1414280714&_rerunOrigin=scholar.google&_acct=C000011279&_version=1&_urlVersion=0&_userid=217827&md5=180870c2ecb405ea5e989cfb7a4293a6 Monitoring of conformational change in maltose binding protein using split green fluorescent proteinstar, open ]