Difference between revisions of "IGEM:Imperial/2010/Output module"

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m (removing outdated task assignments)
(Added Bilins link)
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   ! Effector 1 (Protease) !! Effector 2  (Dye,Enz) !! Pigment biosynthetic pathways
 
   ! Effector 1 (Protease) !! Effector 2  (Dye,Enz) !! Pigment biosynthetic pathways
 
|-
 
|-
   | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless || Billins
+
   | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless || [[IGEM:Imperial/2010/Fast_Response_module/Bilins | Bilins]]
 
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|-
 
   | [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]]
 
   | [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]]

Revision as of 06:03, 20 July 2010

Effector 1 (Protease) Effector 2 (Dye,Enz) Pigment biosynthetic pathways
transcr sigma 54 2 colourless Bilins
Activation (phosphorylation) Enz-in-pathway
  • short pathways
  • ensure - colourless -> colour
Anthocyanins
  • short pathways
  • ensure - colourless -> colour
Target proteases to use
  • not many AA
  • non-toxic
  • can work in E.Coli
  • quantize speed, efficiency
Protein scaffold Fret pairs as back up for effectors
2C DNA binding prot release

This review article has some useful information on FRET.


James- some suggested papers:

  1. Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 | PubMed ID:17117150 | HubMed [1]
  2. Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 | PubMed ID:17072307 | HubMed [2]
All Medline abstracts: PubMed | HubMed