IGEM:Imperial/2010/Output module: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
m (removing outdated task assignments) |
(Added Bilins link) |
||
Line 4: | Line 4: | ||
! Effector 1 (Protease) !! Effector 2 (Dye,Enz) !! Pigment biosynthetic pathways | ! Effector 1 (Protease) !! Effector 2 (Dye,Enz) !! Pigment biosynthetic pathways | ||
|- | |- | ||
| [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless || | | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless || [[IGEM:Imperial/2010/Fast_Response_module/Bilins | Bilins]] | ||
|- | |- | ||
| [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]] | | [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]] |
Revision as of 07:03, 20 July 2010
Effector 1 (Protease) | Effector 2 (Dye,Enz) | Pigment biosynthetic pathways |
---|---|---|
transcr sigma 54 | 2 colourless | Bilins |
Activation (phosphorylation) | Enz-in-pathway
|
Anthocyanins
|
Target proteases to use
|
Protein scaffold | Fret pairs as back up for effectors |
2C DNA binding prot release |
This review article has some useful information on FRET.
James- some suggested papers:
- Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 |
- Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 |