Difference between revisions of "IGEM:Imperial/2010/Output module"

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(FRET article)
(Moving effectors from fast response page to output page)
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{| class="wikitable" style="text-align: center; width: 20%; height: 170px;" border="1"
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{| class="wikitable" style="text-align: center; width: 50%; height: 170px;" border="1"
  
|+ Table of ideas
 
 
|-
 
|-
   ! Pigment biosynthetic pathways
+
   ! Effector 1 (Protease) !! Effector 2  (Dye,Enz) !! Pigment biosynthetic pathways
 
|-
 
|-
   | Billins
+
   | [[IGEM:Imperial/2010/Fast_Response_module/transcr_sigma54 | transcr sigma 54]] || 2 colourless || Billins
 
|-
 
|-
    
+
   | [[IGEM:Imperial/2010/Fast_Response_module/Activation_phosphorylation|Activation (phosphorylation)]]
 +
  | Enz-in-pathway
 +
 
 +
* short pathways
 +
* ensure - colourless -> colour
 
   | Anthocyanins
 
   | Anthocyanins
 
 
* short pathways  
 
* short pathways  
 
* ensure - colourless -> colour
 
* ensure - colourless -> colour
 
|-
 
|-
 
+
  | [[IGEM:Imperial/2010/Fast_Response_module/Target_Proteases | Target proteases ]] to use: Piotr, Nick, Kyasha
 +
* not many AA
 +
* non-toxic
 +
* can work in E.Coli
 +
* quantize speed, efficiency
 +
  | Protein scaffold: Maddie
 
   |Fret pairs as back up for effectors
 
   |Fret pairs as back up for effectors
 
+
|-
 +
  | 2C DNA binding prot release || ||
 
|}
 
|}
  
 
This [http://www.springerlink.com/content/q34u8r7r7u76h045/fulltext.pdf review article] has some useful information on FRET.
 
This [http://www.springerlink.com/content/q34u8r7r7u76h045/fulltext.pdf review article] has some useful information on FRET.
 +
 +
 +
James- some suggested papers:
 +
<biblio>
 +
#1 pmid=17117150
 +
#2 pmid=17072307
 +
</biblio>

Revision as of 03:38, 19 July 2010

Effector 1 (Protease) Effector 2 (Dye,Enz) Pigment biosynthetic pathways
transcr sigma 54 2 colourless Billins
Activation (phosphorylation) Enz-in-pathway
  • short pathways
  • ensure - colourless -> colour
Anthocyanins
  • short pathways
  • ensure - colourless -> colour
Target proteases to use: Piotr, Nick, Kyasha
  • not many AA
  • non-toxic
  • can work in E.Coli
  • quantize speed, efficiency
Protein scaffold: Maddie Fret pairs as back up for effectors
2C DNA binding prot release

This review article has some useful information on FRET.


James- some suggested papers:

  1. Kerppola TK. Complementary methods for studies of protein interactions in living cells. Nat Methods. 2006 Dec;3(12):969-71. DOI:10.1038/nmeth1206-969 | PubMed ID:17117150 | HubMed [1]
  2. Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, and Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods. 2006 Dec;3(12):985-93. DOI:10.1038/nmeth967 | PubMed ID:17072307 | HubMed [2]
All Medline abstracts: PubMed | HubMed