Difference between revisions of "IGEM:Imperial/2010/Movie Night"

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{{Imperial2010/Header}}
 
* A Knight's Tale
 
* A Knight's Tale
 
* Kirill: Gone in 60 seconds
 
* Kirill: Gone in 60 seconds
 +
* Piotr: 21
 +
* Ben: Win a date with Tad Hamilton/The Notebook (or Gattaca)
 +
*Nicolas: 22 :P
 +
 +
=Characterization of XylE=
 +
==Identification of optimun wavelength for measuring absorbance==
 +
[[Image:200-600nm spectr.jpg|thumb||300px|center|]]
 +
Spectra of XylE transformed E.coli after addition of catechol assay. The '''broad peak around 380nm wavelength''' arises is due to the presence of the product of the enzymatic reaction involving pyrocatechol and XylE enzyme. This peak if absent if a culture of XylE transformed cells are measured without the addition of catechol
 +
 +
==Kinetics of XylE enzyme==
 +
[[Image:Michaelis-Menten Curve for XylE.jpg|thumb|400px|left]]
 +
[[Image:Lineweaver Plot for XylE.jpg|thumb|400px|right]]
 +
 +
==Comparative Kinetics of XylE enzyme and GFP-XylE fusion protein==
 +
[[Image:XylE Vs Gfp-XylE 1.jpg|thumb|400px|left]]
 +
[[Image:XylE Vs Gfp-XylE 2.jpg|thumb|400px|right]]

Latest revision as of 16:58, 14 October 2010

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  • A Knight's Tale
  • Kirill: Gone in 60 seconds
  • Piotr: 21
  • Ben: Win a date with Tad Hamilton/The Notebook (or Gattaca)
  • Nicolas: 22 :P

Characterization of XylE

Identification of optimun wavelength for measuring absorbance

200-600nm spectr.jpg

Spectra of XylE transformed E.coli after addition of catechol assay. The broad peak around 380nm wavelength arises is due to the presence of the product of the enzymatic reaction involving pyrocatechol and XylE enzyme. This peak if absent if a culture of XylE transformed cells are measured without the addition of catechol

Kinetics of XylE enzyme

Michaelis-Menten Curve for XylE.jpg
Lineweaver Plot for XylE.jpg

Comparative Kinetics of XylE enzyme and GFP-XylE fusion protein

XylE Vs Gfp-XylE 1.jpg
XylE Vs Gfp-XylE 2.jpg