Difference between revisions of "IGEM:Imperial/2010/Modelling"

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(Experiments for the Protein Display Model)
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=[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Objectives Objectives]=
 
These are our daily objectives.
 
 
=Output Amplification Model=
 
 
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Michaelis_Menten Model based on Michaelis Menten Kinetics]==
 
Comparison between different amplification models (HIV1 and TEV) based on Michaelis Menten kinetics. However, Michaelis Menten kinetics does not apply to our system. Therefore, it had to be modelled from first principle (see below using law of mass action).
 
 
{| class="wikitable" style="text-align: center; width: 100%; height: 170px;"
 
| [[Image:Slide2.JPG|200px|thumb|center|alt=A|Model using HIV1]]
 
| [[Image:TEV.jpg|200px|thumb|center|alt=A|Model using TEV]]
 
|}
 
 
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Mass_Action Model based on Law of Mass Action]==
 
Comparison between these 3 different models (which are based on the law of mass action): Simple production, 1-step and 2-step amplification
 
{| class="wikitable" style="text-align: center; width: 100%; height: 170px;"
 
|[[Image:Simple_production.JPG|200px|thumb|center|alt=A|Simple production of Dioxygenase]]
 
|[[Image:1-step_amplification.JPG|200px|thumb|center|alt=A|1-step amplification]]
 
|[[Image:2-step_amplification.JPG|200px|thumb|center|alt=A|2-step amplification]]
 
|}
 
 
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables1 Variables and Constants for this Model]==
 
Here are the variables and constants that are used in the Output Amplification Model.
 
 
=Protein Display Model=
 
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Protein_Display Protein Display Model]==
 
This is a model showing when the ComD receptor will be activated (after proteins have been cleaved).
 
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables2 Variables and constants for this Model]==
 
Here are the variables and constants that are used in the Protein Display Model.
 
 
=Feedback from Wetlab=
 
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments1 Experiments for the Output Amplification Model]==
 
==[http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments2 Experiments for the Protein Display Model]==
 
 
 
 
 
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Revision as of 08:16, 8 September 2010

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} </style> </head> <body> <div id="wrapper"> <div class="accordionButton"><b>Objectives</b></div> <div class="accordionContent",><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Objectives"><b>Here are our daily objectives.</b></a><br /><br /></div>

<div class="accordionButton"><b>Output Amplification Model</b></div> <div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Michaelis_Menten"><b>Model based on Michaelis Menten Kinetics</b></a><br />Comparison between different amplification models (HIV1 and TEV) based on Michaelis Menten kinetics. However, Michaelis Menten kinetics does not apply to our system. Therefore, it had to be modelled from first principle (see below using law of mass action).<br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Michaelis_Menten#HIV1"><img src="http://www.openwetware.org/images/0/03/Slide2.JPG" height="150" width="200" alt="Model using HIV1"/></a> <a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Michaelis_Menten#TEV"><img src="http://www.openwetware.org/images/4/48/TEV.jpg" height="150" width="200" alt="Model using TEV"/></a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Mass_Action"><b>Model based on Law of Mass Action</b></a><br />Comparison between these 3 different models: Simple production, 1-step and 2-step amplification.<br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Mass_Action#Model_preA:_Simple_Production_of_Dioxygenase"><img src="http://www.openwetware.org/images/7/7f/Simple_production.JPG" height="100" width="300" alt="Simple Production"/></a> <a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Mass_Action#Model_A:_Activation_of_Dioxygenase_by_TEV_enzyme"><img src="http://www.openwetware.org/images/1/1c/1-step_amplification.JPG" height="100" width="300" alt="1-step amplification"/></a> <a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Mass_Action#Model_B:_Activation_of_Dioxygenase_by_TEV_or_activated_split_TEV_enzyme"><img src="http://www.openwetware.org/images/0/02/2-step_amplification.JPG" height="100" width="300" alt="2-step amplification"/></a><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables1"><b>Variables and Constants</b></a><br />Here are the variables and constants that are used in the Protein Display Model.<br /><br /></div>

<div class="accordionButton"><b>Protein Display Model</b></div> <div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Protein_Display"><b>Protein Display Model</b></a><br />This is a model showing when the ComD receptor will be activated (after proteins have been cleaved).<br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Variables2"><b>Variables and Constants</b></a><br />Here are the variables and constants that are used in the Protein Display Model.<br /><br /></div>

<div class="accordionButton"><b>Feedback from Wetlab</b></div> <div class="accordionContent"><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Experiments1"><b>Experiments for Output Amplification Model</b></a><br /><br /><a href="http://www.openwetware.org/wiki/IGEM:Imperial/2010/Modelling"><b>Experiments for Protein Display Model</b></a><br /><br /></div> </div> </body> </html>