Difference between revisions of "IGEM:IMPERIAL/2008/New/Protocols"

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= '''Protocols''' =
 
  
{| cellpadding="1" style="background:#66aadd; border: 1px solid #66aadd; color:black" width=45%
 
!width="100%"|
 
  
<font size=4px><font color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''
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{{Imperial/Box2|Experiments & Protocols|
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Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for ''B. subtilis'' manipulation explicitly, as other teams have previously had difficulty in this area.
 +
 
 +
These protocols worked for us, so any future teams looking to use ''subtilis'' could save quite a bit of time by looking here before diving into the literature!}}
 +
 
 +
 
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{| cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="center" width=50%
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|width="100%"| <font size=4px color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''</font>
 
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*[[/Transformation_protocol_4 | Protocol 2]]
 
*[[/Transformation_protocol_4 | Protocol 2]]
 
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|style="background:#66aadd"| <font size=4px color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''</font>
<font size=4px><font color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''
 
 
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|style="background:#FFFFFF"|  
 
*Protocol
 
*Protocol
 
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|style="background:#66aadd"| <font size=4px color=white>'''3. Transformation Protocol ''E.coli'''''</font>
<font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''
 
 
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*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
 
*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
 
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|style="background:#66aadd"| <font size=4px color=white>'''4. Motility Analysis'''</font>
<font size=4px><font color=white>'''4. Motility Analysis'''
 
 
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*[[/Protocols#Motility_Experiments | Motility Assays]]
 
*[[/Protocols#Motility_Experiments | Motility Assays]]
 
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|style="background:#66aadd"| <font size=4px color=white>'''5. PCR'''</font>
<font size=4px><font color=white>'''5. PCR'''
 
 
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*[[/Colony_PCR| Single Colony PCR]]
 
*[[/Colony_PCR| Single Colony PCR]]
 
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|style="background:#66aadd"| <font size=4px color=white>'''6. Calibration Curves'''</font>
<font size=4px><font color=white>'''6. Calibration Curves'''
 
 
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*[[/Fluorescence| Fluorescence vs GFPmut3b]]
 
*[[/Fluorescence| Fluorescence vs GFPmut3b]]
 
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|style="background:#66aadd"| <font size=4px color=white>'''7. Promoter-RBS Testing Protocols'''</font>
<font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''
 
 
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*[[/Light_Inducible | Light Inducible Promoters and RBS]]
 
*[[/Light_Inducible | Light Inducible Promoters and RBS]]
 
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|style="background:#66aadd"| <font size=4px color=white>'''8. Biomaterial Protocols'''</font>
<font size=4px><font color=white>'''8. Biomaterial Protocols'''
 
 
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*Protocol
 
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<br clear="all"><br><hr>
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{{Imperial/EndPage|Cloning_Strategy|Major_Results}}

Latest revision as of 05:20, 30 September 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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Experiments & Protocols

Below you will find a comprehensive list of all the protocols we used during our wet lab work. We spent some time toward the start of the project sourcing and updating effective protocols for B. subtilis manipulation explicitly, as other teams have previously had difficulty in this area.

These protocols worked for us, so any future teams looking to use subtilis could save quite a bit of time by looking here before diving into the literature!



1. Transformation Protocols for integration vectors in Bacillus subtilis
2. Transformation Protocol for linear DNA in Bacillus subtilis
  • Protocol
3. Transformation Protocol E.coli
4. Motility Analysis
5. PCR
6. Calibration Curves
7. Promoter-RBS Testing Protocols
8. Biomaterial Protocols
  • Protocol




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