Difference between revisions of "IGEM:IMPERIAL/2008/New/Protocols"

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m (Replacing page with '{{Imperial/StartPage2}} = Protocols = {{Protocols}} {{Imperial/EndPage|Cloning_Strategy|Major_Results}}')
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= Protocols =
 
= Protocols =
  
{| cellpadding="1" style="background:#66aadd; border:1px solid #66aadd; color:black" align="left" width=50%
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{{Protocols}}
|width="100%"| <font size=4px><font color=white>'''1. Transformation Protocols for integration vectors in ''Bacillus subtilis'''''
 
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|style="background:#FFFFFF"|
 
*[[/Transformation_protocol_2 | Protocol 1]]
 
*[[/Transformation_protocol_4 | Protocol 2]]
 
|-
 
|style="background:#66aadd"| <font size=4px><font color=white>'''2. Transformation Protocol for linear DNA in ''Bacillus subtilis'''''
 
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|style="background:#FFFFFF"|
 
*Protocol
 
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|style="background:#66aadd"| <font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''
 
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|style="background:#FFFFFF"|
 
*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
 
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|style="background:#66aadd"| <font size=4px><font color=white>'''4. Motility Analysis'''
 
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|style="background:#FFFFFF"|
 
*[[/Protocols#Motility_Experiments | Motility Assays]]
 
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|style="background:#66aadd"| <font size=4px><font color=white>'''5. PCR'''
 
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|style="background:#FFFFFF"|
 
*[[/PCR| PCR]]
 
*[[/Colony_PCR| Single Colony PCR]]
 
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|style="background:#66aadd"| <font size=4px><font color=white>'''6. Calibration Curves'''
 
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|style="background:#FFFFFF"|
 
*[[/Calibration-Curves| Calibration Curves ]]
 
*[[/Growth| O.D.<sub>600</sub> vs cell number]]
 
*[[/Fluorescence| Fluorescence vs GFPmut3b]]
 
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|style="background:#66aadd"| <font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''
 
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|style="background:#FFFFFF"|
 
*[[/Constitutive | Constitutive Promoters and RBS]]
 
*[[/Inducible | pHyperspank Promoter and RBS]]
 
*[[/Light_Inducible | Light Inducible Promoters and RBS]]
 
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|style="background:#66aadd"| <font size=4px><font color=white>'''8. Biomaterial Protocols'''
 
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|style="background:#FFFFFF"|
 
*Protocol
 
|}
 
<table><tr><td>
 
  
 
{{Imperial/EndPage|Cloning_Strategy|Major_Results}}
 
{{Imperial/EndPage|Cloning_Strategy|Major_Results}}

Revision as of 05:43, 18 September 2008

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Protocols

OpenWetware wg.jpg

In vivo

Escherichia coli

{{{ecoli}}}

Yeast

{{{yeast}}}

T7

{{{T7}}}

Mesoplasma florum

{{{meso}}}

In vitro

Nucleic acids

{{{nucleic acids}}}

DNA

{{{DNA}}}

RNA

{{{RNA}}}

Protein

{{{Protein}}}

Other

Microfluidics

{{{microfluidics}}}

Microscopy

{{{microscopy}}}

Flow Cytometry

{{{cytometry}}}

Plate reader

{{{plate reader}}}



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