Difference between revisions of "IGEM:IMPERIAL/2008/New/Protocols"

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*[[/Transformation_protocol_2 | Protocol 1]]
 
*[[/Transformation_protocol_2 | Protocol 1]]
 
*[[/Transformation_protocol_4 | Protocol 2]]
 
*[[/Transformation_protocol_4 | Protocol 2]]
 
 
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<font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''
 
<font size=4px><font color=white>'''3. Transformation Protocol ''E.coli'''''
 
 
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*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
 
*[[/XL1-Blue_preparation | Preparation of XL1-blue electrocompetent cells]]
 
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<font size=4px><font color=white>'''4. Motility Analysis'''
 
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4. Motility Analysis
 
 
*[[/Protocols#Motility_Experiments | Motility Assays]]
 
*[[/Protocols#Motility_Experiments | Motility Assays]]
5. PCR
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<font size=4px><font color=white>'''5. PCR'''
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*[[/PCR| PCR]]
 
*[[/PCR| PCR]]
 
*[[/Colony_PCR| Single Colony PCR]]
 
*[[/Colony_PCR| Single Colony PCR]]
6. Calibration Curves<br>
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<font size=4px><font color=white>'''6. Calibration Curves'''
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*[[/Calibration-Curves| Calibration Curves ]]
 
*[[/Calibration-Curves| Calibration Curves ]]
 
*[[/Growth| O.D.<sub>600</sub> vs cell number]]
 
*[[/Growth| O.D.<sub>600</sub> vs cell number]]
 
*[[/Fluorescence| Fluorescence vs GFPmut3b]]
 
*[[/Fluorescence| Fluorescence vs GFPmut3b]]
7. Promoter-RBS Testing Protocols<br>
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<font size=4px><font color=white>'''7. Promoter-RBS Testing Protocols'''
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*[[/Constitutive | Constitutive Promoters and RBS]]  
 
*[[/Constitutive | Constitutive Promoters and RBS]]  
 
*[[/Inducible | pHyperspank Promoter and RBS]]
 
*[[/Inducible | pHyperspank Promoter and RBS]]
 
*[[/Light_Inducible | Light Inducible Promoters and RBS]]
 
*[[/Light_Inducible | Light Inducible Promoters and RBS]]
8. Biomaterial Protocols
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<font size=4px><font color=white>'''8. Biomaterial Protocols'''
 
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Revision as of 15:09, 11 September 2008

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Protocols

1. Transformation Protocols for integration vectors in Bacillus subtilis

2. Transformation Protocol for linear DNA in Bacillus subtilis

3. Transformation Protocol E.coli

4. Motility Analysis

5. PCR

6. Calibration Curves

7. Promoter-RBS Testing Protocols

8. Biomaterial Protocols


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