Difference between revisions of "IGEM:IMPERIAL/2008/New/Project"

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{{Imperial/StartPage2}} __NOTOC__
 
{{Imperial/StartPage2}} __NOTOC__
=Biofabricator Subtilis=
 
 
[[Image:Engineering_Cycle.png|right|thumb|Our design process]]
 
  
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&nbsp; <font size=6px color=#E5EBFF><b>Biofabricator Subtilis</b></font>
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<br>
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<br>
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{| cellpadding="1" style="background:#2B48B3; border:4px solid #99BBFF; color:#E5EBFF" align="center" width=90%
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In designing our ''Biofabricator Subtilis'', we followed the engineering approach for synthetic biology.  
 
In designing our ''Biofabricator Subtilis'', we followed the engineering approach for synthetic biology.  
  
  
We started with a set of basic specifications our project must meet in order to fulfill our aim of designing a genetically-engineering biofabricator. Since there are three main parts to our project, our specifications can be broadly classified into three categories - light sensing, motility control and biomaterial synthesis.  
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We started with a set of basic specifications our project must meet in order to fulfill our aim of designing a genetically-engineering biofabricator. Since there are three main parts to our project, our specifications can be broadly classified into three categories - light sensing, motility control and biomaterial synthesis.
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[[Image:Imperial_2008_Engineering_Cycle.png|right|250px|Our design process]]
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|}
 
<br clear="all">
 
<br clear="all">
  
==Our Specifications==
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&nbsp; <font size=6px color=#E5EBFF><b>Project Specifications</b></font>
 
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<br>
 
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<br>
===Light Sensing===
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{| cellpadding="1" style="background:#E5EBFF; border:4px solid #2B48B3; color:#2B48B3" align="center" width=90%
[[Image:Imperial_2008_Light_Sensing.jpg|right|thumb]]
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|colspan="2"|<font size=5px color=#2B48B3>Light Sensing</font>
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|-
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* The photoreceptor must have a short response time, preferably under one minute.
 
* The photoreceptor must have a short response time, preferably under one minute.
 
* The light sensing pathway must be able to be coupled to an inducible promoter, to allow control of our downstream systems (motility control and biomaterial production).
 
* The light sensing pathway must be able to be coupled to an inducible promoter, to allow control of our downstream systems (motility control and biomaterial production).
 
* The photoreceptor must be compatible with the chassis.
 
* The photoreceptor must be compatible with the chassis.
 
* The pathway must not give rise to negative secondary effects such as interference with native pathways.
 
* The pathway must not give rise to negative secondary effects such as interference with native pathways.
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[[Image:Imperial_2008_Light_Sensing.png|right|250px]]
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|}<br clear="all">
  
===Motility Control===
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{| cellpadding="1" style="background:#E5EBFF; border:4px solid #2B48B3; color:#2B48B3" align="center" width=90%
[[Image:Imperial_2008_Motility.jpg|right|thumb]]
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|colspan="2"|<font size=5px color=#2B48B3>Motility Control</font>
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|-
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* Quick and effective control of bacteria motility - we should be able to stop bacteria locomotion efficiently.
 
* Quick and effective control of bacteria motility - we should be able to stop bacteria locomotion efficiently.
 
* The mechanism for motility control should be reversible - bacteria should be able to regain locomotion after a certain recovery period, but this is not essential.
 
* The mechanism for motility control should be reversible - bacteria should be able to regain locomotion after a certain recovery period, but this is not essential.
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[[Image:Imperial_2008_Motility.png|right|250px|A clutch... Like, from a car?]]
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|}<br clear="all">
  
===Biomaterial Production===
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{| cellpadding="1" style="background:#E5EBFF; border:4px solid #2B48B3; color:#2B48B3" align="center" width=90%
[[Image:Imperial_2008_Moebius_Gear.JPG|right|thumb]]
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|colspan="2"|<font size=5px color=#2B48B3>Biomaterial Production</font>
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* Bacteria should be able to secrete large amounts of a self-assembling biomaterial into the growth medium.
 
* Bacteria should be able to secrete large amounts of a self-assembling biomaterial into the growth medium.
 
* Biomaterial must be small enough to facilitate its secretion by the chassis.  
 
* Biomaterial must be small enough to facilitate its secretion by the chassis.  
* Biomaterial should be compatible with tissue engineering or regenerative medicine.
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* Biomaterial should be compatible with tissue engineering or regenerative medicine.<br clear="all">
 
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[[Image:Imperial_2008_Moebius_Gear.png|right|250px|The Moebius Gear, produced by 3D printing]]
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|}
  
 
<br clear="all"><hr>
 
<br clear="all"><hr>
  
 
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<font color=#E5EBFF>
For more information on how ''B. subtilis'' meets our specifications and why we have embarked on the ambitious route of using it as our chassis, please refer to the next page.  [[IGEM:IMPERIAL/2008/New/Chassis_1 | '''>>> Why B. subtilis? >>>''']]
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{{Imperial/Box1||For more information on how ''B. subtilis'' meets our specifications and why we have embarked on the ambitious route of using it as our chassis, please refer to the next page.  [[IGEM:IMPERIAL/2008/New/Chassis_1 | '''>>> Why B. subtilis? >>>''']]</font>}}
 
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{{Imperial/EndPage|Home|Chassis_1}}
 
 
{{Imperial/EndPage}}
 

Latest revision as of 06:13, 27 September 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Genetic_Circuit">Genetic Circuits</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Motility">Motility Analysis</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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  Biofabricator Subtilis

In designing our Biofabricator Subtilis, we followed the engineering approach for synthetic biology.


We started with a set of basic specifications our project must meet in order to fulfill our aim of designing a genetically-engineering biofabricator. Since there are three main parts to our project, our specifications can be broadly classified into three categories - light sensing, motility control and biomaterial synthesis.

Our design process


  Project Specifications

Light Sensing
  • The photoreceptor must have a short response time, preferably under one minute.
  • The light sensing pathway must be able to be coupled to an inducible promoter, to allow control of our downstream systems (motility control and biomaterial production).
  • The photoreceptor must be compatible with the chassis.
  • The pathway must not give rise to negative secondary effects such as interference with native pathways.
Imperial 2008 Light Sensing.png

Motility Control
  • Quick and effective control of bacteria motility - we should be able to stop bacteria locomotion efficiently.
  • The mechanism for motility control should be reversible - bacteria should be able to regain locomotion after a certain recovery period, but this is not essential.
A clutch... Like, from a car?

Biomaterial Production
  • Bacteria should be able to secrete large amounts of a self-assembling biomaterial into the growth medium.
  • Biomaterial must be small enough to facilitate its secretion by the chassis.
  • Biomaterial should be compatible with tissue engineering or regenerative medicine.
The Moebius Gear, produced by 3D printing


For more information on how B. subtilis meets our specifications and why we have embarked on the ambitious route of using it as our chassis, please refer to the next page. >>> Why B. subtilis? >>>



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