IGEM:IMPERIAL/2008/New/Growth Curve: Difference between revisions
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{{Imperial/Box2|Growth Curve| | {{Imperial/Box2|Growth Curve| | ||
Part of our dry lab team concentrated on modelling the growth curve of ''B. subtilis''. This is important to characterise the chassis; particularly, the growth of ''subtilis'' is a vital parameter for planning experiments for future projects. | Part of our dry lab team concentrated on modelling the growth curve of ''B. subtilis''. This is important to characterise the chassis; particularly, the growth of ''subtilis'' is a vital parameter for planning experiments for future projects. Characterisation increases the predictability of the growth of B. subtilis by determining, for example, its growth rate and the duration of its distinctive growth phases. In order to model the growth of ''B. subtilis'', the process was broken down into three main steps, where a separate submodel is produced in MATLAB for each step. Each submodel is an ODE model, which can be simulated using MATLAB. The variables in each submodel can be adjusted according to the boundary conditions (from experimental results). | ||
In the final step, a combination of Submodels 1 and 2 are superposed with Submodel 3, resulting in a more complex model which enhances the accuracy of illustrating bacterial growth. For more details about the submodels, please click on the following links: | |||
'''[[Tutorial for Growth Curve]]''' (More details about each submodel) | |||
'''[[Media:Modelling_Growth_Curve.pdf]]''' (The tutorial questions) | |||
The graph on the right shows the log graph used to determine the growth rate. | The graph on the right shows the log graph used to determine the growth rate. | ||
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{{Imperial/Box1|The Model| | |||
The model illustrates the main growth phases the ''B. subtilis'' undergoes. These are identified as the '''lag phase''', the '''exponential phase''' and the '''stationary phase'''. The death phase is a constitutive event and it is possible that it exerts an influence on the three phases discussed below. However, to simplify a complicated model, it is less relevant in this case and therefore is not included in this model. | |||
The M-file used to generate the model below is located in the Appendices section, its link can be found at the bottom of this page. | The M-file used to generate the model below is located in the Appendices section, its link can be found at the bottom of this page. | ||
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*Time span of lag phase, stationary phase and exponential phase | *Time span of lag phase, stationary phase and exponential phase | ||
*The growth rate | *The growth rate}} | ||
}} | |||
{{Imperial/Box1|| | {{Imperial/Box1|| |
Revision as of 04:40, 30 September 2008
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