IGEM:IMPERIAL/2008/New/Cloning Strategy

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Authors: Chris

Editors: Wetlab team, wiki team

Cloning Strategy

The Imperial iGEM 2008 team faces the mammoth task of working with a chassis that has been rarely used in the competition so far. This leaves us with many challenges to overcome, in particular having to work from the ground up.

This also makes our cloning stratergy highly complicated. In order to building increasingly complicated constructs for our final product, we must first build and test simpler parts and devices.

Imperial 2008 Critical Pathway.png

This is the overall cloning strategy for our project. A summary of constructs that should be produced by the end of each phase for testing is shown below:

Phase 1
Phase 1.PNG
Phase 2A Phase 2B
Phase 2-linduced.PNG Phase 2-light induced.PNG
Phase 3A Phase 3B
Phase 2-linduced22.PNG Phase 2-biomaterials.PNG
Phase 4A Phase 4B
Phase 4-clutch.PNG Phase 4-clutch.PNG
Final Construct
Imperial 2008 Named Circuitry.jpg