Difference between revisions of "IGEM:IMPERIAL/2008/New/Cloning Strategy"

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= Cloning Strategy =
 
= Cloning Strategy =
  
The Imperial iGEM 2008 team faces the mammoth task of working with a chassis that has been rarely used in the competition so far. This leaves us with many challenges to overcome, in particular having to work from the ground up.  
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The Imperial iGEM 2008 team faces the daunting task of working with a chassis that has been rarely used - and never characterised - in the competition so far. While the ''subtilis'' chassis offers us many advantages, working from the ground up like this also presents many challenges.
  
This also makes our cloning strategy highly complicated. In order to building increasingly complicated constructs for our final product, we must first build and test simpler parts and devices.
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Our cloning strategy, for instance, is highly complex. In order to building increasingly complicated constructs for our final product, we need to build, test and characterise all the parts and devices leading up to the final systems! The diagram below shows the "critical pathway" for our cloning strategy and as you can see, there are a huge number of closely-linked steps..
  
  
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A summary of constructs that should be produced by the end of each phase for testing is shown below:
 
 
This is the overall cloning strategy for our project. A summary of constructs that should be produced by the end of each phase for testing is shown below:
 
  
 
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Revision as of 11:30, 11 September 2008

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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Cloning_Strategy">Cloning Strategy</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols">Experiments & Protocols</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a>
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       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Motility">Motility Analysis</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a>
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</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

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Cloning Strategy

The Imperial iGEM 2008 team faces the daunting task of working with a chassis that has been rarely used - and never characterised - in the competition so far. While the subtilis chassis offers us many advantages, working from the ground up like this also presents many challenges.

Our cloning strategy, for instance, is highly complex. In order to building increasingly complicated constructs for our final product, we need to build, test and characterise all the parts and devices leading up to the final systems! The diagram below shows the "critical pathway" for our cloning strategy and as you can see, there are a huge number of closely-linked steps..


Imperial 2008 Critical Pathway.png


A summary of constructs that should be produced by the end of each phase for testing is shown below:

Phase 1
Phase 1.PNG
Phase 2A Phase 2B
Phase 2-linduced.PNG Phase 2-light induced.PNG
Phase 3A Phase 3B
Phase 2-linduced22.PNG Phase 2-biomaterials.PNG
Phase 4A Phase 4B
Phase 4-clutch.PNG Phase 4-clutch.PNG
Final Construct
Imperial 2008 Named Circuitry.jpg