IGEM:IMPERIAL/2008/New/Chassis 2: Difference between revisions
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= Benefits | <font size=6px color=#E5EBFF><b>Benefits vs Challenges</b></font> | ||
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{| cellpadding="5" style="background:#66aadd; border: 4px solid #66aadd; color:black" align="center" width= | {| cellpadding="5" style="background:#66aadd; border: 4px solid #66aadd; color:black" align="center" width=95% | ||
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!width="50%"| | !width="50%"| | ||
<font size=4px | <font size=4px color=white><center>'''Benefits'''</center></font> | ||
!width="50%"|<font size=4px | !width="50%"|<font size=4px color=white><center>'''Challenges'''</center></font> | ||
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|style="background:#99BBFF" colspan=" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" colspan="2"|<font size=3px color=white><center>'''Protein Expression'''</center></font> | |||
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|style="background:#FFFFFF"| [[Image:Secretion.PNG| 150px]] | |style="background:#FFFFFF"| [[Image:Secretion.PNG| 150px]] | ||
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|style="background:#FFFFFF"| '''Peptidase activity''' - ''B. subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted. | |style="background:#FFFFFF"| '''Peptidase activity''' - ''B. subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted. | ||
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|style="background:#99BBFF" colspan=" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" colspan="2"|<font size=3px color=white><center>'''Foundational Technology'''</center></font> | |||
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|rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]] | |rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]] | ||
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|style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B. subtilis'' as a potential chassis, there are very few working ''B. subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | |style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B. subtilis'' as a potential chassis, there are very few working ''B. subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | ||
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|style="background:#99BBFF" colspan=" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" colspan="2"|<font size=3px color=white><center>'''BioBrick Assembly'''</center></font> | |||
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|style="background:#FFFFFF"|[[Image:Integration.PNG| 150px]] | |style="background:#FFFFFF"|[[Image:Integration.PNG| 150px]] | ||
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|style="background:#FFFFFF"| '''Vector Degradation''' - ''B. subtilis'' does not use all the same vectors as ''E. coli''. One reason for this is that ''B. subtilis'' often recognises vectors grown in ''E. coli'' as foreign and digests them. Vectors and shuttle vectors are thus not reliable carriers of genetic information. | |style="background:#FFFFFF"| '''Vector Degradation''' - ''B. subtilis'' does not use all the same vectors as ''E. coli''. One reason for this is that ''B. subtilis'' often recognises vectors grown in ''E. coli'' as foreign and digests them. Vectors and shuttle vectors are thus not reliable carriers of genetic information. | ||
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|style="background:#99BBFF" colspan=" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" colspan="2"|<font size=3px color=white><center>'''Chassis Properties'''</center></font> | |||
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| rowspan=3 style="background:#FFFFFF"| [[Image:Chassis.PNG| 150px]] | | rowspan=3 style="background:#FFFFFF"| [[Image:Chassis.PNG| 150px]] | ||
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|style="background:#FFFFFF"|'''Sporulation: Transport''' - Under stress conditions, ''B. subtilis'' will form spores. These spores are highly resistant versions of single cells, able to withstand extreme temperatures and pH. These spores are capable of growing into new cells once favourable growing conditions are restored. Due to the resistance of spores, there is a great potential for manipulating them for transporting ''B. subtilis'' devices and constructs. | |style="background:#FFFFFF"|'''Sporulation: Transport''' - Under stress conditions, ''B. subtilis'' will form spores. These spores are highly resistant versions of single cells, able to withstand extreme temperatures and pH. These spores are capable of growing into new cells once favourable growing conditions are restored. Due to the resistance of spores, there is a great potential for manipulating them for transporting ''B. subtilis'' devices and constructs. | ||
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<br> | <br clear="all"><hr> | ||
{{Imperial/Box1||Having decided what we're doing and how we plan to do it, the next step is to get started! To see how our lab work went please start with the [[IGEM:IMPERIAL/2008/New/Wet_Lab | '''>>> Wet Lab Hub >>>''']]}} | |||
<hr> | {{Imperial/EndPage|Chassis_1|Wet_Lab}} | ||
Having decided what we're doing and how we plan to do it, the next step is to get started! To see how our lab work went please start with the [[IGEM:IMPERIAL/2008/New/Wet_Lab | '''>>> Wet Lab Hub >>>''']] |
Latest revision as of 07:14, 27 September 2008
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