IGEM:IMPERIAL/2008/New/Chassis 2: Difference between revisions
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!width="50%"|<font size=4px><font color=white><center>'''Challenges'''</center> | !width="50%"|<font size=4px><font color=white><center>'''Challenges'''</center> | ||
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|style="background:#99BBFF" colspan=" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" colspan="2"|<font size=3px><font color=white><center>'''Protein Expression'''</center> | |||
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|style="background:#FFFFFF"| [[Image:Secretion.PNG| 150px]] | |style="background:#FFFFFF"| [[Image:Secretion.PNG| 150px]] | ||
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|style="background:#FFFFFF"| '''Peptidase activity''' - ''B. subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted. | |style="background:#FFFFFF"| '''Peptidase activity''' - ''B. subtilis'' express exopeptidases and so there is a risk that our biomaterial may be cleaved up before it can be secreted. | ||
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|style="background:#99BBFF" colspan=" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" colspan="2"|<font size=3px><font color=white><center>'''Foundational Technology'''</center> | |||
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|rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]] | |rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]] | ||
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|style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B. subtilis'' as a potential chassis, there are very few working ''B. subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | |style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B. subtilis'' as a potential chassis, there are very few working ''B. subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | ||
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|style="background:#99BBFF" colspan=" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" colspan="2"|<font size=3px><font color=white><center>'''BioBrick Assembly</center> | |||
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|style="background:#FFFFFF"|[[Image:Integration.PNG| 150px]] | |style="background:#FFFFFF"|[[Image:Integration.PNG| 150px]] | ||
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|style="background:#FFFFFF"| '''Vector Degradation''' - ''B. subtilis'' does not use all the same vectors as ''E. coli''. One reason for this is that ''B. subtilis'' often recognises vectors grown in ''E. coli'' as foreign and digests them. Vectors and shuttle vectors are thus not reliable carriers of genetic information. | |style="background:#FFFFFF"| '''Vector Degradation''' - ''B. subtilis'' does not use all the same vectors as ''E. coli''. One reason for this is that ''B. subtilis'' often recognises vectors grown in ''E. coli'' as foreign and digests them. Vectors and shuttle vectors are thus not reliable carriers of genetic information. | ||
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|style="background:#99BBFF" colspan=" | |style="background:#99BBFF"| | ||
|style="background:#99BBFF" colspan="2"|<font size=3px><font color=white><center>'''Chassis Properties'''</center> | |||
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| rowspan=3 style="background:#FFFFFF"| [[Image:Chassis.PNG| 150px]] | | rowspan=3 style="background:#FFFFFF"| [[Image:Chassis.PNG| 150px]] |
Revision as of 14:04, 16 September 2008
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