IGEM:IMPERIAL/2008/New/Chassis 2: Difference between revisions
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|rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]] | |rowspan=2 style="background:#FFFFFF" |[[Image:Gene.PNG| 150px]] | ||
| rowspan=2 style="background:#FFFFFF"| '''Availability of Potential Parts''' - ''B.subtilis'' is a highly studied organism, with a fully sequenced genome. As a result, ''B.subtilis'' provides many useful potential parts. | | rowspan=2 style="background:#FFFFFF"| '''Availability of Potential Parts''' - ''B.subtilis'' is a highly studied organism, with a fully sequenced genome. As a result, ''B.subtilis'' provides many useful potential parts. | ||
|style="background:#FFFFFF"| ''' | |style="background:#FFFFFF"| '''BioBricking''' - Although there is a huge number of potential parts, these still have to be manipulated to conform to existing BioBrick standards. | ||
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|style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B.subtilis'' as a potential chassis, there are very few working ''B.subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | |style="background:#FFFFFF"| '''Starting from scratch''' - Although previous iGEM teams have looked at ''B.subtilis'' as a potential chassis, there are very few working ''B.subtilis'' parts available in the Registry. As a result, we will have to design a variety of promoter, ribosome binding sites and protein coding regions. | ||
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|style="background:#99BBFF" |<font size=3px><font color=white><center>''' | |style="background:#99BBFF" |<font size=3px><font color=white><center>'''BioBrick Assembly</center> | ||
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|style="background:#FFFFFF"| '''Natural Competency and Integration''' - ''B. subtilis'' has been noted for its ease and efficieny of transformation. Plasmids can be naturally taken up by ''B. subtilis'' cells. In addition to the use of vectors to carry exogenous DNA, we can integrate directly into the ''B.subtilis'' chromosome. This has the advantage of a greater control of copy number and potential greater stability of genetic devices. | |style="background:#FFFFFF"| '''Natural Competency and Integration''' - ''B. subtilis'' has been noted for its ease and efficieny of transformation. Plasmids can be naturally taken up by ''B. subtilis'' cells. In addition to the use of vectors to carry exogenous DNA, we can integrate directly into the ''B.subtilis'' chromosome. This has the advantage of a greater control of copy number and potential greater stability of genetic devices. | ||
|style="background:#FFFFFF"| '''Vector Degradation''' - ''B.subtilis'' does not use all the same vectors as ''E.coli''. One reason for this is that ''B.subtilis'' often recognises vectors grown in ''E.coli'' and digests them. This will (judging by the approach of previous iGEM teams) include the | |style="background:#FFFFFF"| '''Vector Degradation''' - ''B.subtilis'' does not use all the same vectors as ''E.coli''. One reason for this is that ''B.subtilis'' often recognises vectors grown in ''E.coli'' and digests them. This will (judging by the approach of previous iGEM teams) include the BioBrick itself. | ||
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|style="background:#99BBFF" width=50%|<font size=3px><font color=white><center>'''Chassis Properties'''</center> | |style="background:#99BBFF" width=50%|<font size=3px><font color=white><center>'''Chassis Properties'''</center> |
Revision as of 03:31, 11 September 2008
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