Difference between revisions of "IGEM:IMPERIAL/2008/New/BioBricks"

From OpenWetWare
Jump to: navigation, search
m
m
 
(22 intermediate revisions by the same user not shown)
Line 34: Line 34:
 
| Ligation pending/Failed
 
| Ligation pending/Failed
 
|- style=background:red
 
|- style=background:red
| Awaiting GeneArt/Cloning
+
| Awaiting GeneArt
 +
|- style=background:maroon
 +
| Cloning difficulties
 
|}
 
|}
 
{| border="2" cellpadding="2" style="text-align:left" align="left" width="75%"
 
{| border="2" cellpadding="2" style="text-align:left" align="left" width="75%"
 
!Part Number !! Part Name !! Status  
 
!Part Number !! Part Name !! Status  
|- style=background:#cc4400
+
|- style=background:gold
| K143001 || 5' AmyE Integration Sequence || Midiprepped
+
| K143001 || 5' AmyE Integration Sequence || Submitted
|- style=background:silver
+
|- style=background:gold
| K143002 || 3' AmyE Integration Sequence || Sequenced
+
| K143002 || 3' AmyE Integration Sequence || Submitted
|- style=background:yellow
+
|- style=background:gold
| K143005 || 5' EpsE Integration Sequence || Awaiting Ligation
+
| K143005 || 5' EpsE Integration Sequence || Submitted
|- style=background:yellow
+
|- style=background:gold
| K143006 || 3' EpsE Integration Sequence || Awaiting Ligation
+
| K143006 || 3' EpsE Integration Sequence || Submitted
|- style=background:red
 
| K143008 || 5' PyrD Integration Sequence || Awaiting GeneArt delivery
 
 
|- style=background:royalblue
 
|- style=background:royalblue
| K143009 || 3' PyrD Integration Sequence || Transformed 1-10
+
| K143008 || 5' PyrD Integration Sequence || Delayed
|- style=background:yellow
+
|- style=background:gold
| K143031 || Aad9 Spectinomycin Resistance Gene || Ligation Failed
+
| K143009 || 3' PyrD Integration Sequence || Submitted
|- style=background:silver
+
|- style=background:gold
| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Sequenced
+
| K143031 || Aad9 Spectinomycin Resistance Gene || Submitted
|- style=background:mediumslateblue
+
|- style=background:gold
| K143033 || LacI (Lva<sup>-</sup>, N terminal deletion) || PCR verified
+
| K143032 || EpsE Molecular Clutch Gene of ''B. subtilis'' || Submitted
|- style=background:#cc4400
+
|- style=background:gold
| K143034 || LipA-EAK16 Fusion || Midiprepped
+
| K143033 || LacI (Lva<sup>-</sup>, N terminal deletion) || Submitted
 +
|- style=background:gold
 +
| K143034 || LipA-EAK16 Fusion || Submitted
 +
|- style=background:gold
 +
| K143035 || LipA-Human Elastin Fusion || Submitted
 
|- style=background:royalblue
 
|- style=background:royalblue
| K143035 || LipA-Human Elastin Fusion || Transformed 25/9
+
| K143036 || Xylose Operon Regulatory Protein || Delayed
 +
|- style=background:gold
 +
| K143037 || Ytva Blue Light Receptor of ''B. subtilis'' || Submitted
 +
|- style=background:gold
 +
| K143038 || SacB-EAK16 Fusion || Submitted
 +
|- style=background:gold
 +
| K143039 || SacB-Human Elastin Fusion || Submitted
 +
|- style=background:gold
 +
| K143050 || P43-gsiB || Submitted
 +
|- style=background:gold
 +
| K143051 || P43-spoVG || Submitted
 +
|- style=background:gold
 +
| K143052 || Pveg-gsiB || Submitted
 +
|- style=background:gold
 +
| K143053 || Pveg-spoVG || Submitted
 +
|- style=background:gold
 +
| K143054 || Phyperspank-gsiB || Submitted
 +
|- style=background:gold
 +
| K143055 || Phyperspank-spoVG || Submitted
 +
|- style=background:gold
 +
| K143056 || Pxyl-gsiB || Submitted
 +
|- style=background:gold
 +
| K143057 || Pxyl-spoVG || Submitted
 +
|- style=background:gold
 +
| K143058 || Pctc-gsiB || Submitted
 +
|- style=background:gold
 +
| K143059 || Pctc-spoVG || Submitted
 +
|- style=background:gold
 +
| K143060 || PgsiB-gsiB || Submitted
 +
|- style=background:gold
 +
| K143061 || PgsiB-spoVG || Submitted
 +
|- style=background:gold
 +
| K143062 || LacI - Terminator || Submitted
 
|- style=background:royalblue
 
|- style=background:royalblue
| K143036 || Xylose Operon Regulatory Protein || Transformed 29/9
+
| K143063 || XylR - Terminator || Delayed
 +
|- style=background:gold
 +
| K143064 || CAT - Terminator || Submitted
 +
|- style=background:gold
 +
| K143065 || Aad9 - Terminator || Submitted
 +
|- style=background:gold
 +
| K143066 || YtvA - Terminator || Submitted
 +
|- style=background:gold
 +
| K143067 || AmyE integratable PoPS generator (P43-gsiB) || Submitted
 +
|- style=background:gold
 +
| K143068 || AmyE integratable PoPS generator (P43-spoVG) || Submitted
 +
|- style=background:gold
 +
| K143069 || AmyE integratable PoPS generator (Pveg-gsiB) || Submitted
 +
|- style=background:gold
 +
| K143070 || AmyE integratable PoPS generator (Pveg-spoVG) || Submitted
 +
|- style=background:gold
 +
| K143071 || AmyE integratable PoPS generator (P43-gsiB) (with CmR) || Submitted
 +
|- style=background:gold
 +
| K143072 || AmyE integratable PoPS generator (P43-spoVG) (with CmR) || Submitted
 
|- style=background:royalblue
 
|- style=background:royalblue
| K143037 || Ytva Blue Light Receptor of ''B. subtilis'' || Transformed 1-10
+
| K143073 || AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) || Delayed
|- style=background:silver
+
|- style=background:gold
| K143038 || SacB-EAK16 Fusion || Sequenced
+
| K143074 || AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) || Submitted
|- style=background:#cc4400
+
|- style=background:gold
| K143050 || P43-gsiB || Midiprepped
+
| K143075 || B. subtilis AmyE locus GFP output promoter and RBS tester || Submitted
|- style=background:#cc4400
+
|- style=background:gold
| K143051 || P43-spoVG || Midiprepped
+
| K143076 || B. subtilis AmyE locus RFP output promoter and RBS tester || Submitted
|- style=background:#cc4400
+
|- style=background:gold
| K143052 || Pveg-gsiB || Midiprepped
+
| K143077 || P43-gsiB GFP expression construct  || Submitted
|- style=background:#cc4400
+
|- style=background:gold
| K143053 || Pveg-spoVG || Midiprepped
+
| K143078 || P43-spoVG GFP expression construct  || Submitted
|- style=background:red
+
|- style=background:gold
| K143054 || Phyperspank-gsiB || Awaiting GeneArt Delivery
+
| K143079 || Pveg-spoVG GFP expression construct  || Submitted
|- style=background:royalblue
+
|- style=background:gold
| K143055 || Phyperspank-spoVG || Transformed 1-10
+
| K143080 || P43-gsiB RFP expression construct  || Submitted
|- style=background:red
+
|- style=background:gold
| K143056 || Pxyl-gsiB || Awaiting GeneArt Delivery
+
| K143081 || P43-spoVG RFP expression construct  || Submitted
|- style=background:red
+
|- style=background:gold
| K143057 || Pxyl-spoVG || Awaiting GeneArt Delivery
+
| K143082 || Pveg-spoVG RFP expression construct  || Submitted
|- style=background:royalblue
 
| K143058 || Pctc-gsiB || Transformed 1-10
 
|- style=background:royalblue
 
| K143059 || Pctc-spoVG || Transformed 1-10
 
|- style=background:#cc4400
 
| K143060 || PgsiB-gsiB || Midiprepped
 
|- style=background:#cc4400
 
| K143061 || PgsiB-spoVG || Midiprepped
 
|- style=background:red
 
| K143062 || LacI - Terminator || Awaiting Components (LacI)
 
|- style=background:red
 
| K143063 || XylR - Terminator || Awaiting Components (XylR)
 
|- style=background:yellow
 
| K143064 || CAT - Terminator || Transformed 1-10
 
|- style=background:red
 
| K143065 || Aad9 - Terminator || Awaiting Components (Aad9)
 
 
|}
 
|}
  
 
{{Imperial/EndPage|Major_Results|Dry_Lab}}
 
{{Imperial/EndPage|Major_Results|Dry_Lab}}

Latest revision as of 18:56, 28 October 2008

<html> <style type="text/css"> .firstHeading {display: none;} </style> </html> <html> <style type="text/css">

   table.calendar          { margin:0; padding:2px; }

table.calendar td { margin:0; padding:1px; vertical-align:top; } table.month .heading td { padding:1px; background-color:#FFFFFF; text-align:center; font-size:120%; font-weight:bold; } table.month .dow td { text-align:center; font-size:110%; } table.month td.today { background-color:#3366FF } table.month td {

   border:2px;
   margin:0;
   padding:0pt 1.5pt;
   font-size:8pt;
   text-align:right;
   background-color:#FFFFFF;
   }
  1. bodyContent table.month a { background:none; padding:0 }

.day-active { font-weight:bold; } .day-empty { color:black; } </style> </html>

<html><script language="JavaScript">

var timeout = 250; var closetimer = 0; var ddmenuitem = 0;

// open hidden layer function mopen(id) { // cancel close timer mcancelclosetime(); // close old layer if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; // get new layer and show it ddmenuitem = document.getElementById(id); ddmenuitem.style.visibility = 'visible'; } // close showed layer function mclose() { if(ddmenuitem) ddmenuitem.style.visibility = 'hidden'; } // go close timer function mclosetime() { closetimer = window.setTimeout(mclose, timeout); } // cancel close timer function mcancelclosetime() { if(closetimer) { window.clearTimeout(closetimer); closetimer = null; } } // close layer when click-out //document.onclick = mclose; </script> <table background="http://i59.photobucket.com/albums/g305/Timpski/ToolbarBackground.png" style="color:#ffffff;" link="#ffffff" cellpadding="0" cellspacing="1" border="0" bordercolor="#ffffff" align="center" width="100%"><tr><td colspan="6" class="wetlab"><br><br><br></td></tr> <tr><td align="center" width="10%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Home"> Home </a></ul> </td><td align="center" width="20%" valign="bottom"><ul id="sddm"><a href="#"

       onclick="mopen('m1')" 
       onmouseover="mopen('m1')" 
       onmouseout="mclosetime()">Biofabricator Subtilis</a>
       <div id="m1" 
           onmouseover="mcancelclosetime()" 
           onmouseout="mclosetime()">
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Project">Project Specifications</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_1">Why B. subtilis?</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Chassis_2">B. subtilis: Benefits vs Challenges</a>
       </div></ul>

</td><td align="center" width="18%" valign="bottom"><ul id="sddm"><a href="#"

       onclick="mopen('m2')" 
       onmouseover="mopen('m2')" 
       onmouseout="mclosetime()">Wet Lab</a>
       <div id="m2" 
           onmouseover="mcancelclosetime()" 
           onmouseout="mclosetime()">
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Wet_Lab">Wet Lab Hub</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Cloning_Strategy">Cloning Strategy</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Protocols">Experiments & Protocols</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Major_Results">Experimental Results</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/BioBricks">BioBricks & Characterisation</a>
       </div></ul>

</td><td align="center" width="18%" valign="bottom"><ul id="sddm"><a href="#"

       onclick="mopen('m3')" 
       onmouseover="mopen('m3')" 
       onmouseout="mclosetime()">Dry Lab</a>
       <div id="m3" 
           onmouseover="mcancelclosetime()" 
           onmouseout="mclosetime()">
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Dry_Lab">Dry Lab Hub</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Growth_Curve">Growth Curves</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Genetic_Circuit">Genetic Circuits</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Motility">Motility Analysis</a>
       <a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Appendices">Appendices - Code etc.</a>
       </div></ul>

</td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://2008.igem.org/Team:Imperial_College/Notebook"> Notebook </a></ul> </td><td align="center" width="17%" valign="bottom"><ul id="sddm"><a href="http://openwetware.org/wiki/IGEM:IMPERIAL/2008/New/Team"> Our Team </a></ul> </td></tr></table></html>

<html><style type="text/css">

div.Section { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; background-image: url(http://openwetware.org/images/a/a0/Background.PNG); background-size: 100%; background-origin: content; }

/* Text (paragraphs) */ div.Section p { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:justify; margin-top:0px; margin-left:30px; margin-right:30px; }

/* Headings */ div.Section h1 { font:22pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:left; color:#3366FF; font-weight:bold; }

/* Subheadings */ div.Section h2 { font:18pt Calibri, Verdana, Arial, Geneva, sans-serif; color:#3366FF; margin-left:5px; font-weight:bold; }

/* Subsubheadings */ div.Section h3 { font:22pt Calibri, Verdana, Arial, sans-serif; color:#E5EBFF; margin-left:10px; font-weight:bold; }

/* Subsubsubheadings */ div.Section h4 { font:22pt Calibri, Verdana, Arial, sans-serif; color:#2B48B3; margin-left:10px; font-weight:bold; }

/* Subsubsubsubheadings */ div.Section h5 { font:12pt Calibri, Verdana, Arial, sans-serif; color:#3366FF; margin-left:20px; }

/* References */ div.Section h6 { font:12pt Calibri, Verdana, Arial, sans-serif; font-weight:bold; font-style:italic; color:#3366FF; margin-left:25px; }

/* Hyperlinks */ div.Section a { }

div.Section a:hover { }

/* Tables */ div.Section td { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:justify; vertical-align:top; padding:2px 4px 2px 4px; }

/* Lists */ div.Section li { font:11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align:left; margin-top:0px; margin-left:30px; margin-right:0px; }

/* TOC stuff */ table.toc { margin-left:10px; }

table.toc li { font: 11pt/16pt Calibri, Verdana, Arial, Geneva, sans-serif; text-align: justify; margin-top: 0px; margin-left:2px; margin-right:2px; }

/* [edit] links */ span.editsection { color:#BBBBBB; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

span.editsection a { color:#BBBBBB; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

span.editsection a:hover { color:#3366FF; font-size:10pt; font-weight:normal; font-style:normal; vertical-align:bottom; }

/* Drop-down Menu */

  1. sddm {

margin: 0; padding: 0; z-index: 30 margin: 0; padding: 0; float: center; font: bold 12pt Calibri, Verdana, Arial, Geneva, sans-serif; border: 0px; list-style: none; }

  1. sddm a {

display: block; margin: 0px 0px 0px 0px; padding: 0 0 12px 0; color: #FFFFFF; text-align: center; text-decoration: none; }

  1. sddm a:hover {

border: 0px }

  1. sddm div {

position: absolute; visibility: hidden; margin: 0; padding: 0; background: #66aadd; border: 1px solid #66aadd } #sddm div a { position: relative; left: 0; display: block; margin: 0; padding: 5px 10px; width: auto; white-space: nowrap; text-align: left; text-decoration: none; background: #FFFFFF; color: #2875DE; font: 11pt Calibri, Verdana, Arial, Geneva, sans-serif } #sddm div a:hover { background: #66aadd; color: #FFFFFF } </style></html>


BioBricks & Characterisation

Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.

Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.

<html><center><img width="100%" src="http://i59.photobucket.com/albums/g305/Timpski/S1L.png">| Device 1 | Device 2 | Device 3 |<br>| The System |</center></html>


Parts Submitted to the Registry

For now, here are the parts we will submit to the Registry. They can also be accessed here.

<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Imperial_College" width="100%" height="600px" scrolling="yes"></iframe></html>


Production Status

This table shows our progress with each part:


Key
Submitted
Sequenced
Midiprepped
Fully verified
Partially Verified
Transformed
Ligation pending/Failed
Awaiting GeneArt
Cloning difficulties
Part Number Part Name Status
K143001 5' AmyE Integration Sequence Submitted
K143002 3' AmyE Integration Sequence Submitted
K143005 5' EpsE Integration Sequence Submitted
K143006 3' EpsE Integration Sequence Submitted
K143008 5' PyrD Integration Sequence Delayed
K143009 3' PyrD Integration Sequence Submitted
K143031 Aad9 Spectinomycin Resistance Gene Submitted
K143032 EpsE Molecular Clutch Gene of B. subtilis Submitted
K143033 LacI (Lva-, N terminal deletion) Submitted
K143034 LipA-EAK16 Fusion Submitted
K143035 LipA-Human Elastin Fusion Submitted
K143036 Xylose Operon Regulatory Protein Delayed
K143037 Ytva Blue Light Receptor of B. subtilis Submitted
K143038 SacB-EAK16 Fusion Submitted
K143039 SacB-Human Elastin Fusion Submitted
K143050 P43-gsiB Submitted
K143051 P43-spoVG Submitted
K143052 Pveg-gsiB Submitted
K143053 Pveg-spoVG Submitted
K143054 Phyperspank-gsiB Submitted
K143055 Phyperspank-spoVG Submitted
K143056 Pxyl-gsiB Submitted
K143057 Pxyl-spoVG Submitted
K143058 Pctc-gsiB Submitted
K143059 Pctc-spoVG Submitted
K143060 PgsiB-gsiB Submitted
K143061 PgsiB-spoVG Submitted
K143062 LacI - Terminator Submitted
K143063 XylR - Terminator Delayed
K143064 CAT - Terminator Submitted
K143065 Aad9 - Terminator Submitted
K143066 YtvA - Terminator Submitted
K143067 AmyE integratable PoPS generator (P43-gsiB) Submitted
K143068 AmyE integratable PoPS generator (P43-spoVG) Submitted
K143069 AmyE integratable PoPS generator (Pveg-gsiB) Submitted
K143070 AmyE integratable PoPS generator (Pveg-spoVG) Submitted
K143071 AmyE integratable PoPS generator (P43-gsiB) (with CmR) Submitted
K143072 AmyE integratable PoPS generator (P43-spoVG) (with CmR) Submitted
K143073 AmyE integratable PoPS generator (Pveg-gsiB) (with CmR) Delayed
K143074 AmyE integratable PoPS generator (Pveg-spoVG) (with CmR) Submitted
K143075 B. subtilis AmyE locus GFP output promoter and RBS tester Submitted
K143076 B. subtilis AmyE locus RFP output promoter and RBS tester Submitted
K143077 P43-gsiB GFP expression construct Submitted
K143078 P43-spoVG GFP expression construct Submitted
K143079 Pveg-spoVG GFP expression construct Submitted
K143080 P43-gsiB RFP expression construct Submitted
K143081 P43-spoVG RFP expression construct Submitted
K143082 Pveg-spoVG RFP expression construct Submitted


<html><center><table style="color:#ffffff;background-color:#66aadd;" cellpadding="3" cellspacing="1" border="0" bordercolor="#ffffff" align="center"> <tr><td><ul id="sddm"></html>< Previous<html></ul> </td><td><ul id="sddm"><a href="#">Back to top</a></ul> </td><td><ul id="sddm"></html>Next ><html></ul> </td></tr></table> </center></html>