IGEM:IMPERIAL/2007/Tutorials/Guide for Engineers/Standard Lab Techniques

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The Molecular Biologist Toolbox Kit

Restriction Enzymes

Restriction enzymes are a class of enzyme that can causes specific cuts in a DNA molecule.The mechanism by which restriction enzymes cut DNA is by causing hydrolysis of the phosphodiester bonds between the nucleotides that make up DNA.
Restriction enzymes can be broadly split into two types:

  • Exonucleases cleave DNA from the ends of the DNA molecule. The action of exonucleases will remove nucleotides from the ends of DNA.
  • Endonucleases cleave within the interior of the DNA molecule. The action of endonucleases will cause fragmentation of DNA molecules.

DNA polymerase

Vectors and Plasmids

Gene Cloning

Gel Electrophoresis

Blotting Techniques

Western Blotting

Southern Blotting

Northern Blotting

PCR (Polymerase Chain Reaction)

DNA Sequencing