Difference between revisions of "IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes"

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(Biofilm Detection: Notes)
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==Making Biofilm==
 
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*Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.  
 
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*Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
 
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*Add 1 mL of E. coli solution to each well.  
==Project Goals==
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*The 24-well plate is immediately covered and transferred to a 30 °C incubator.
*Calibrate basic construct F2620 in ''E. coli''
 
*Get basic construct F2620 working ''in vitro''
 
*Describe and model differences of construct ''in vivo'' and ''in vitro''
 
 
 
*Build final construct with β-galactosidase
 
*Calibrate final construct with β-galactosidase in ''E. coli''
 
*Get basic construct with β-galactosidase working ''in vitro''
 
*Model the construct ''in vivo'' and ''in vitro''
 
*Optimize construct for biofilm detection
 
 
 
*Model the possible function of Hrp in the system
 
*Test Hrp in the system
 
 
 
*Create small amount of biofilm using ''E. coli''
 
 
 
==Project Status==
 
'''Protocols:'''
 
*''E. coli'' testing protocols written
 
*''in vitro'' protocols being refined
 
 
 
'''Wet Lab:'''
 
*Test plasmid under cloning (ready at Week 7)
 
*Bricks for final constructs awaiting ligation
 
 
 
'''Modelling:'''
 
*AHL concentration on plux is currently being modelled
 

Revision as of 03:50, 11 September 2007

Infector Detector: Notes



Making Biofilm

  • Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.
  • Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
  • Add 1 mL of E. coli solution to each well.
  • The 24-well plate is immediately covered and transferred to a 30 °C incubator.