Difference between revisions of "IGEM:IMPERIAL/2007/Notebook/2007-9-10"

From OpenWetWare
m
 
Line 17: Line 17:
  
 
Three POPC/dodecane 10ml suspensions were prepared.
 
Three POPC/dodecane 10ml suspensions were prepared.
 +
 +
==Cloning of Biobricks (for Maxiprep)==
 +
#Placed 100 ml of LB in a 500 ml conical flask
 +
#Innoculated 1 ml of culture into LB media in flask
 +
#Incubated overnight at 37 °C
 +
 +
<font size=-2>
 +
*25. BBa_J37032 [pLux-GFP]
 +
*13. BBa_B0015 [ ]
 +
</font>
 +
 +
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep|Maxiprep]] in the general protocols page
 +
 +
==Transformation of Biobricks==
 +
#1 biobrick was transformed into XL1-Blue
 +
#Cells were grown for half an hour and plated
 +
#Plates were incubated at 37&deg;C overnight
 +
 +
*Transformed in XL1-Blue<font size=-2>
 +
**pSB1A3 [pSB1A3] (re-clone)</font>
 +
 +
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electroporation|Electroporation]] in the general protocols page

Latest revision as of 06:29, 11 September 2007

<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/10 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>


Vesicles

Results

Sample 8, produced on 04.09.2007 was looked at again under the fluorescence microscope, but this time only a single 2μm vesicle was found.

Preparations

Three POPC/dodecane 10ml suspensions were prepared.

Cloning of Biobricks (for Maxiprep)

  1. Placed 100 ml of LB in a 500 ml conical flask
  2. Innoculated 1 ml of culture into LB media in flask
  3. Incubated overnight at 37 °C

  • 25. BBa_J37032 [pLux-GFP]
  • 13. BBa_B0015 [ ]

Protocols can be found at Maxiprep in the general protocols page

Transformation of Biobricks

  1. 1 biobrick was transformed into XL1-Blue
  2. Cells were grown for half an hour and plated
  3. Plates were incubated at 37°C overnight
  • Transformed in XL1-Blue
    • pSB1A3 [pSB1A3] (re-clone)

Protocols can be found at Electroporation in the general protocols page