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Cre-LoxP System


The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part J37015. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch controlled by the activity of the promoter in front of the Cre enzyme.

  • Using Cre recombinase as a switch appears to be, in theory, a very efficient and solid method.

Part interface

(describe your black-box approach, define properties of inputs/outputs)

input 1 output 1
input 2 output 2
input 3
IGEM IMPERIAL Methodology Specifications.png

(don't forget to insert a relevant image of your black box.)

Expected behavior and performances

(try to write your specifications in tables. Here are just 2 examples)

Interface specifiactions
Characteristics Input 1 Input 2 Input 3 Output 1 Output 2
Range [X1,Y1] [X2,Y2] [X3,Y3] [X4,Y4] [X5,Y5]
Tolerance T1 T2 T3 T4 T5
Transfer function n/a n/a n/a insert image insert image

System level specifications
Properties Comments
Transfer function
properties 3
properties 4

Open issues