IGEM:IMPERIAL/2006/project/popsblocker: Difference between revisions
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==Motivations== | ==Motivations== | ||
*The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part [http://parts.mit.edu/registry/index.php?title=Part:BBa_J37015 J37015]. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme. | |||
In theory, using Cre recombinase as a switch/Pops blocker appears to be a very efficient and solid method. | *In theory, using Cre recombinase as a switch/Pops blocker appears to be a very efficient and solid method. | ||
==Part interface== | ==Part interface== | ||
Revision as of 13:39, 26 October 2006
Cre-LoxP System
Motivations
- The use of Cre recombinase to excise regions of DNA between two loxP sites could be incorporated into part J37015. Our aim is to insert two loxP sites into this part, which, when in place, would block the synthesis of any genes downstream of it, and when removed would allow their synthesis. Thus the Cre-loxP system would effectively act as a switch/Pops blocker controlled by the activity of the promoter in front of the Cre enzyme.
- In theory, using Cre recombinase as a switch/Pops blocker appears to be a very efficient and solid method.
Part interface
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