IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Implementation: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
No edit summary
Line 37: Line 37:
|}
|}


*More information on ligations can be found on the [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Ligation_Page Ligation Page].


==Quality control==
==Quality control==

Revision as of 20:28, 29 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Prey Construct <bbpart>C0261</bbpart> <bbpart>I13401</bbpart>


Ligation Strategy

Ligating the Prey Construct:

For Stage 1 Stage 2
<bbpart>J37034</bbpart>=
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)
<bbpart>J37015</bbpart>=
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)


Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37034</bbpart>
Yes ? ?
<bbpart>J37015</bbpart>
Yes ? ?

WetLab work entries

  • Part has been successfully built.
  • Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.