IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct: Difference between revisions

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==Motivation==
==Motivation==


The motivation to build this part is to characterise the positive feedback loop in the prey cell, and from this be able to characterise (and possibly extend) the exponetial phase of AHL production.
*The '''Prey Construct''' must control the molecular activity associated with the Prey in the '''[[IGEM:IMPERIAL/2006/project/Oscillator/Theoretical_Analyses/2D_Model1|Lotka-Volterra model]]'''.
*This part has to interface with the '''[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Predator_Construct Predator-construct]'''.
*To satisfy the conditions for Lotka-Volterra, this part has to produce the molecular prey exponentially.
 


==Part Interface==
==Part Interface==
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[[Image:iGEM_IMPERIAL_Methodology_Specifications.png|right|350px]]
[[Image:Preyblackbox.png|right|350px]]
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==Expected behavior and performances==
 
{| border="1" width="100%"
System level specifications
|-
! style="background:lightgrey" width="100pt" |Properties !! colspan="2" | Specifications
|-
| Input Range(s)|| colspan="2" | Input range should be sufficient to produce a detectable output of prey.
|-
|Output Range(s)|| colspan="2" | Output range of the prey should be detectable and sufficient to interact with the predator.
|-
| Expected Output || [[Image:AHLvTime.png|200px]]
To satisfy Lotka-Volterra conditions, our system should produce prey exponentially.
|-
| Robustness || colspan="2" | Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.
|-
| Variability  || colspan="2" | Variability at the device population level (day to day, device to device) should be as low as possible.
|}
|}

Revision as of 17:29, 29 October 2006

Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Prey Construct <bbpart>C0261</bbpart> <bbpart>I13401</bbpart>



Motivation

  • The Prey Construct must control the molecular activity associated with the Prey in the Lotka-Volterra model.
  • This part has to interface with the Predator-construct.
  • To satisfy the conditions for Lotka-Volterra, this part has to produce the molecular prey exponentially.


Part Interface

INPUTS OUTPUTS
AHL AHL

Expected behavior and performances

System level specifications
Properties Specifications
Input Range(s) Input range should be sufficient to produce a detectable output of prey.
Output Range(s) Output range of the prey should be detectable and sufficient to interact with the predator.
Expected Output

To satisfy Lotka-Volterra conditions, our system should produce prey exponentially.

Robustness Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.
Variability Variability at the device population level (day to day, device to device) should be as low as possible.
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