IGEM:IMPERIAL/2006/Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
mNo edit summary |
||
| (8 intermediate revisions by one other user not shown) | |||
| Line 8: | Line 8: | ||
</div> | </div> | ||
<br style="clear:both"> | <br style="clear:both"> | ||
==Protocols== | ==Protocols== | ||
*[[IGEM:IMPERIAL/2006 | *[[IGEM:IMPERIAL/2006/Protocols/LB_Amp_plate|LB Amp Plate]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/transformation|Transformations]] | |||
*[[IGEM:IMPERIAL/Protocols/transformation|Transformations]] | *[[IGEM:IMPERIAL/2006/Protocols/competent_cells_DH5a|Competent Cells]] | ||
*[[IGEM:IMPERIAL/Protocols/competent_cells_DH5a|Competent Cells]] | *[[IGEM:IMPERIAL/2006/Protocols/bradford_assay|Bradford Assay]] | ||
*[[IGEM:IMPERIAL/Protocols/bradford_assay|Bradford Assay]] | *[[IGEM:IMPERIAL/2006/Protocols/20ul_digestion|20 {{uL}} Digestion]] | ||
*[[IGEM:IMPERIAL/Protocols/20ul_digestion|20 uL Digestion]] | *[[IGEM:IMPERIAL/2006/Protocols/20ul_ligation|20 {{uL}} Ligation]] | ||
*[[IGEM:IMPERIAL/Protocols/20ul_ligation|20 uL Ligation]] | *[[IGEM:IMPERIAL/2006/Protocols/30ul_ligation|30 {{uL}} Ligation]] | ||
*[[IGEM:IMPERIAL/Protocols/30ul_ligation|30 uL Ligation]] | *[[IGEM:IMPERIAL/2006/Protocols/mini_prep_of_culture|Miniprep of Culture]] | ||
*[[IGEM:IMPERIAL/Protocols/mini_prep_of_culture|Miniprep of Culture]] | *[[IGEM:IMPERIAL/2006/Protocols/50X_TAE|50x TAE Solution]] | ||
*[[IGEM:IMPERIAL/Protocols/50X_TAE|50x TAE Solution]] | *[[IGEM:IMPERIAL/2006/Protocols/agarose_gel|Agarose Gel]] | ||
*[[IGEM:IMPERIAL/Protocols/agarose_gel|Agarose Gel]] | *[[IGEM:IMPERIAL/2006/Protocols/measuring_dna_concentration|Measuring DNA Concentration]] | ||
*[[IGEM:IMPERIAL/Protocols/measuring_dna_concentration|Measuring DNA Concentration]] | *[[IGEM:IMPERIAL/2006/Protocols/fusion PCR|Fusion PCR Protocol]] | ||
*[[IGEM:IMPERIAL/Protocols/fusion PCR|Fusion PCR | *[[IGEM:IMPERIAL/2006/Protocols/western_blot|Western Blot Protocol]] | ||
*[[IGEM:IMPERIAL/Protocols/western_blot|Western Blot Protocol]] | |||
*[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]] | *[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]] | ||
*[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria) | *[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria) | ||
| Line 32: | Line 29: | ||
*[http://openwetware.org/images/f/f5/PCRv.miniprep2.ppt PCR v. miniprep (flow chart)] | *[http://openwetware.org/images/f/f5/PCRv.miniprep2.ppt PCR v. miniprep (flow chart)] | ||
<html> | |||
<!-- Start of StatCounter Code --> | |||
<script type="text/javascript" language="javascript"> | |||
var sc_project=1999441; | |||
var sc_invisible=1; | |||
var sc_partition=18; | |||
var sc_security="18996820"; | |||
</script> | |||
<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&java=0&security=18996820&invisible=1" alt="website statistics" border="0"></a> </noscript> | |||
<!-- End of StatCounter Code --> | |||
</html> | |||
Latest revision as of 09:50, 30 October 2006
Protocols
- LB Amp Plate
- Transformations
- Competent Cells
- Bradford Assay
- 20 μL Digestion
- 20 μL Ligation
- 30 μL Ligation
- Miniprep of Culture
- 50x TAE Solution
- Agarose Gel
- Measuring DNA Concentration
- Fusion PCR Protocol
- Western Blot Protocol
- 1 kb Plus DNA Ladder
- Freezing cells (Protocol from OWW: Making a long term stock of bacteria)
<html>
<!-- Start of StatCounter Code -->
<script type="text/javascript" language="javascript">
var sc_project=1999441;
var sc_invisible=1;
var sc_partition=18;
var sc_security="18996820";
</script>
<script type="text/javascript" language="javascript" src="http://www.statcounter.com/counter/frames.js"></script><noscript><a href="http://www.statcounter.com/" target="_blank"><img src="http://c19.statcounter.com/counter.php?sc_project=1999441&java=0&security=18996820&invisible=1" alt="website statistics" border="0"></a> </noscript> <!-- End of StatCounter Code --> </html>
