IGEM:IMPERIAL/2006/Protocols: Difference between revisions
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<li>[[IGEM:IMPERIAL/2006/Protocols |General Protocols]]</li> | |||
<li>[[IGEM:IMPERIAL/2006/Protocols/Testing |Testing Protocols]]</li> | |||
</ul> | |||
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==Protocols== | ==Protocols== | ||
*[[IGEM:IMPERIAL/ | *[[IGEM:IMPERIAL/2006/Protocols/LB_Amp_plate|LB Amp Plate]] | ||
*[[IGEM:IMPERIAL/2006/Protocols/transformation|Transformations]] | |||
*[[IGEM:IMPERIAL/Protocols/transformation|Transformations]] | *[[IGEM:IMPERIAL/2006/Protocols/competent_cells_DH5a|Competent Cells]] | ||
*[[IGEM:IMPERIAL/Protocols/competent_cells_DH5a|Competent Cells]] | *[[IGEM:IMPERIAL/2006/Protocols/bradford_assay|Bradford Assay]] | ||
*[[IGEM:IMPERIAL/Protocols/bradford_assay|Bradford Assay]] | *[[IGEM:IMPERIAL/2006/Protocols/20ul_digestion|20 {{uL}} Digestion]] | ||
*[[IGEM:IMPERIAL/Protocols/20ul_digestion|20 uL Digestion]] | *[[IGEM:IMPERIAL/2006/Protocols/20ul_ligation|20 {{uL}} Ligation]] | ||
*[[IGEM:IMPERIAL/Protocols/20ul_ligation|20 uL Ligation]] | *[[IGEM:IMPERIAL/2006/Protocols/30ul_ligation|30 {{uL}} Ligation]] | ||
*[[IGEM:IMPERIAL/Protocols/30ul_ligation|30 uL Ligation]] | *[[IGEM:IMPERIAL/2006/Protocols/mini_prep_of_culture|Miniprep of Culture]] | ||
*[[IGEM:IMPERIAL/Protocols/mini_prep_of_culture|Miniprep of Culture]] | *[[IGEM:IMPERIAL/2006/Protocols/50X_TAE|50x TAE Solution]] | ||
*[[IGEM:IMPERIAL/Protocols/50X_TAE|50x TAE Solution]] | *[[IGEM:IMPERIAL/2006/Protocols/agarose_gel|Agarose Gel]] | ||
*[[IGEM:IMPERIAL/Protocols/agarose_gel|Agarose Gel]] | *[[IGEM:IMPERIAL/2006/Protocols/measuring_dna_concentration|Measuring DNA Concentration]] | ||
*[[IGEM:IMPERIAL/Protocols/measuring_dna_concentration|Measuring DNA Concentration]] | *[[IGEM:IMPERIAL/2006/Protocols/fusion PCR|Fusion PCR Protocol]] | ||
*[[IGEM:IMPERIAL/Protocols/fusion PCR|Fusion PCR | *[[IGEM:IMPERIAL/2006/Protocols/western_blot|Western Blot Protocol]] | ||
*[[IGEM:IMPERIAL/Protocols/western_blot|Western Blot Protocol | |||
*[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]] | *[[Kafatos:DNA 1kbplus ladder|1 kb Plus DNA Ladder]] | ||
*[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria) | *[http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria Freezing cells] (Protocol from OWW: Making a long term stock of bacteria) | ||
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*[http://openwetware.org/images/f/f5/PCRv.miniprep2.ppt PCR v. miniprep (flow chart)] | *[http://openwetware.org/images/f/f5/PCRv.miniprep2.ppt PCR v. miniprep (flow chart)] | ||
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Latest revision as of 09:50, 30 October 2006
Protocols
- LB Amp Plate
- Transformations
- Competent Cells
- Bradford Assay
- 20 μL Digestion
- 20 μL Ligation
- 30 μL Ligation
- Miniprep of Culture
- 50x TAE Solution
- Agarose Gel
- Measuring DNA Concentration
- Fusion PCR Protocol
- Western Blot Protocol
- 1 kb Plus DNA Ladder
- Freezing cells (Protocol from OWW: Making a long term stock of bacteria)
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