Difference between revisions of "IGEM:Harvard/2010/Plasmids"
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Revision as of 12:48, 21 June 2010
the plant vectors we will be biobrick-ing and using to transform into arabidopsis come from this paper:
Coutu, et al. "pORE: a modular binary vector series suited for both monocot and dicot plant transformation" Transgenic Res (2007) 16: 771-781.
this paper established a whole slew of vectors with different plant promoters and antibiotic resistance markers.
we have ordered the following vectors: O1, O2, E3, E4, R1, and R3.
these plasmids currently contain multiple cloning sites (MCSs) with many, many restriction enzyme cut sites. we will need to change the MCSs such that they are biobrick compatible.