Difference between revisions of "IGEM:Harvard/2010/Brainstorming/food"

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*[http://partsregistry.org/IGEM:MIT/2006/AL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid, MIT iGEM  2006]
*[http://partsregistry.org/IGEM:MIT/2006/AL (Phenylalanine Ammonia-lyase) catalyzes the conversion of phenylalanine to cinnamic acid, MIT iGEM  2006]
*[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-4HYD5PG-5&_user=10&_coverDate=01%2F23%2F2006&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=3a7e5c42ef7261e48ea4e9b523d8312a Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce]
*[http://jb.oxfordjournals.org.ezp-prod1.hul.harvard.edu/cgi/reprint/145/4/445 Functional Expression of Miraculin, a Taste-Modifying Protein in Escherichia Coli]

Revision as of 11:56, 3 May 2010

Strains, Plasmids, and Trasformation


1. Broothearts et. al. Gene transfer to plants by diverse species of bacteria. Nature, 2004.

2. Galvin [1] Agrobacterium-Mediated Plant Transformation: the Biology behind the “Gene-Jockeying” Tool

Agrobacterium infection diagram

3. Antunes, et. al. [2] A synthetic de-greening gene circuit provides a reporting system that is remotely detectable and has a re-set capacity



  1. Chuang CF and Meyerowitz EM. Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4985-90. DOI:10.1073/pnas.060034297 | PubMed ID:10781109 | HubMed [1]

Markers and Kill Switches

Dual selection system (aka killswitch) ideas

  • use selectable markers such as URA3 to control whether an integration plasmid is replicated or not
  • cells with the URA3 gene can grow on media without uracil, but get killed by 5-Fluoroorotic acid (5-FOA)
  • cells without the URA3 gene die on media without uracil, but can survive exposure to 5-FOA

Downstream effectors


  1. Singh MB and Bhalla PL. Genetic engineering for removing food allergens from plants. Trends Plant Sci. 2008 Jun;13(6):257-60. DOI:10.1016/j.tplants.2008.04.004 | PubMed ID:18467156 | HubMed [1]
  2. Dodo H, Konan K, and Viquez O. A genetic engineering strategy to eliminate peanut allergy. Curr Allergy Asthma Rep. 2005 Jan;5(1):67-73. PubMed ID:15659267 | HubMed [2]

All Medline abstracts: PubMed | HubMed


  • [3] Tieman et al. Tomato aromatic amino acid decarboxylases participate in synthesis of the flavor volatiles 2-phenylethanol and 2-phenylacetaldehyde


  1. Theerasilp S and Kurihara Y. Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit. J Biol Chem. 1988 Aug 15;263(23):11536-9. PubMed ID:3403544 | HubMed [1]
  1. Sinha AK, Sharma UK, and Sharma N. A comprehensive review on vanilla flavor: extraction, isolation and quantification of vanillin and others constituents. Int J Food Sci Nutr. 2008 Jun;59(4):299-326. DOI:10.1080/09687630701539350 | PubMed ID:17886091 | HubMed [2]