Difference between revisions of "IGEM:Harvard/2009/Notebook/Harvard iGEM 2010/2010/08/09"

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(Team Allergy)
(Team Allergy)
Line 7: Line 7:
 
==Team Allergy==
 
==Team Allergy==
 
#Redigest V9/V10 (three digests of each backbone to use more DNA in ligations)
 
#Redigest V9/V10 (three digests of each backbone to use more DNA in ligations)
#Redo ihpRNA ligations into V9/V10
+
#Grew up V9/V10+ ihpRNA colonies
#amiRNA transformations into V0120
+
#Re pcrd amiRNA and digested to ligate into V9/V10
#Catalog parts  
+
#Catalog parts to be ready for the registry
  
 
==Team Fence==
 
==Team Fence==

Revision as of 09:07, 10 August 2010

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Team Allergy

  1. Redigest V9/V10 (three digests of each backbone to use more DNA in ligations)
  2. Grew up V9/V10+ ihpRNA colonies
  3. Re pcrd amiRNA and digested to ligate into V9/V10
  4. Catalog parts to be ready for the registry

Team Fence

Redo of some digests with no insert where there should have been an insert File:89moredigestspm.jpg